Results 211 to 220 of about 246,465 (258)
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A fluorescence video-endoscopy technique for detection of gene transfer and expression
Gene Therapy, 1998The green fluorescent protein (GFP) has previously been adapted as a reported for gene transfer and expression in mammalian cells in culture and in tissue sections. Herein is described a new method for detecting GFP in situ within epithelia accessible to fiberoptic endoscopy by incorporating fluorescent filters for detection of GFP into an existing ...
T R, Flotte +5 more
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Gene Transfer Techniques and their relevance to Woody Plants
2000For decades, the concept of foreign DNA transfer into plant cells and its integration into the genome of the recipient cell was an intriguing curiosity (Lurquin, 1977). It was not until the advent of Southern hybridization (Southern, 1975) and the availability of restriction enzymes that the presence of `non-plant’ DNA (in this case, the T-DNA of ...
Minocha, Subhash C., Wallace, John C.
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Gene Transfer with DNA Strand Technique and Peripheral Nerve Injuries
Journal of Long-Term Effects of Medical Implants, 2002Although much progress has been made, current treatments of peripheral nerve injury often result in only partial recovery. Many methods have been used to improve the surgical exploration for injured nerve repair. However, much less is known about the involvement of complex neurochemical interactions. Several neurotrophic factors have been shown to play
Feng, Zhang, William C, Lineaweaver
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Plasmid-mediated gene transfer in neurons using the biolistics technique
Journal of Neuroscience Methods, 1997Biolistics has been developed as a system for gene delivery into plant cells, but has recently been introduced for transfection into mammalian tissue, including few attempts in neural cells. Basically, in this system the plasmid DNA of interest is coated onto small particles, that are accelerated by a particular driving force.
J E, Biewenga +2 more
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Techniques of Chromosome-Mediated Gene Transfer
1982Functional genes can be transferred to eukaryotic cells by incubation with isolated metaphase chromosomes. Uptake occurs by phagocytosis and most of the ingested chromosomal DNA is degraded to small inactive fragments. The frequency of transfer of any selected marker ranges from about 10-5 to 10-7.
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Prospects and Possibilities for Gene Transfer Techniques in Insects
1993The ability to analyze and manipulate organisms by classical genetic means has proven to be one of the most powerful tools for studying basic biological questions as well as for addressing important problems in agriculture and medicine. Today we have -available a new set of genetic tools, those of molecular genetics, which allow researchers to more ...
David A. O’Brochta, Alfred M. Handler
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Development of a Gene Transfer Technique Using AFM
AIP Conference Proceedings, 2003We report on a new low invasive technique for gene transfer of living cells using an ultra thin needle, termed a nano‐needle, comprising part of an etched atomic force microscope (AFM) tip. A silicon AFM tip was sharpened using focused ion beam etching to create an etched Si tip with a small diameter (100 to 200 nm).
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Lipospermine-mediated gene transfer technique into murine cultured cortical cells
Journal of Neuroscience Methods, 1997In order to transfer exogenous DNA into embryonic cortical cells, we have chosen a transfection technique using a synthetic lipospermine (dipalmitoylphosphatidylethanolamylspermine, DPPES) which complexes DNA molecules and allows their penetration into the intracellular compartment.
Sola, Brigitte +4 more
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Gene transfer to plants: approaches and available techniques
1993Gene transfer to plants is hampered by a variety of biological problems: the plant cell wall is a perfect barrier and trap for DNA molecules; egg cells, sperm cells and zygotes, are virtually inaccessible; proembryos are extremely small and enclosed within solid tissues; the tiny cells of hidden meristems which contribute to the ‘germline’ may not be ...
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