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Gene Transfer Techniques and their relevance to Woody Plants
2000For decades, the concept of foreign DNA transfer into plant cells and its integration into the genome of the recipient cell was an intriguing curiosity (Lurquin, 1977). It was not until the advent of Southern hybridization (Southern, 1975) and the availability of restriction enzymes that the presence of `non-plant’ DNA (in this case, the T-DNA of ...
Minocha, Subhash C., Wallace, John C.
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Development of a Gene Transfer Technique Using AFM
AIP Conference Proceedings, 2003We report on a new low invasive technique for gene transfer of living cells using an ultra thin needle, termed a nano‐needle, comprising part of an etched atomic force microscope (AFM) tip. A silicon AFM tip was sharpened using focused ion beam etching to create an etched Si tip with a small diameter (100 to 200 nm).
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Methods and Role of Embryo Rescue Technique in Alien Gene Transfer
2013Embryo abortion occurs frequently in wide crosses, and thus embryo rescue is required for survival of the next generation. Rescues are performed by either directly transferring the excised embryo to an artificial medium or indirectly through flower (ovary), immature seed (fertilized ovule), or pod (silique) culture.
Lulsdorf, Monika M. +3 more
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2020
Biotechnological methods for targeted gene transfers into plants are key for successful breeding in the twenty-first century and thus essential for the survival of humanity. Two decades ago, genetic transformation of crop plants was not routine, and it was all but impossible with important cereals such as barley and wheat.
Jafargholi, Imani, Karl-Heinz, Kogel
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Biotechnological methods for targeted gene transfers into plants are key for successful breeding in the twenty-first century and thus essential for the survival of humanity. Two decades ago, genetic transformation of crop plants was not routine, and it was all but impossible with important cereals such as barley and wheat.
Jafargholi, Imani, Karl-Heinz, Kogel
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Techniques of DNA-Mediated Gene Transfer for Eukaryotic Cells
1982DNA transformation in bacteria has permitted detailed genetic analysis as well as the implementation of recombinant DNA technology. The utility of manipulation and cloning of genes in bacteria is well recognized. This chapter will concern the manipulation and cloning of genes in eukaryotic systems ranging from yeast to animal systems. Gene manipulation
Arthur P. Bollon, Saul J. Silverstein
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Growth Factors and Gene Transfer with DNA Strand Technique in Tendon Healing
Journal of Long-Term Effects of Medical Implants, 2002Growth factors are likely to be critically involved in all aspects of tendon healing and many studies, in vitro and in vivo, have tried to define the role of growth factors in this process, and to determine appropriate strategies for their use in tissue engineering to improve the healing of tendons.
Feng, Zhang, William C, Lineaweaver
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A fluorescence video-endoscopy technique for detection of gene transfer and expression
Gene Therapy, 1998The green fluorescent protein (GFP) has previously been adapted as a reported for gene transfer and expression in mammalian cells in culture and in tissue sections. Herein is described a new method for detecting GFP in situ within epithelia accessible to fiberoptic endoscopy by incorporating fluorescent filters for detection of GFP into an existing ...
T R, Flotte +5 more
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Gene Transfer Techniques in Whole Embryo Cultured Post-implantation Mouse Embryos
2013Gene transfer techniques such as electroporation and lipofection are powerful systems for investigating gene function. In this chapter we focus on the methods and applications of gene transfer into specific cells and tissues of post-implantation mouse embryos.
Daisuke, Sakai, Paul A, Trainor
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Gene transfer to plants: approaches and available techniques
1993Gene transfer to plants is hampered by a variety of biological problems: the plant cell wall is a perfect barrier and trap for DNA molecules; egg cells, sperm cells and zygotes, are virtually inaccessible; proembryos are extremely small and enclosed within solid tissues; the tiny cells of hidden meristems which contribute to the ‘germline’ may not be ...
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Gene Transfer Techniques to Study Neuropeptide Processing
Annual Review of Physiology, 1988G, Thomas, B A, Thorne, D E, Hruby
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