Results 71 to 80 of about 365,940 (292)

Two-phase stretching of molecular chains

, 2013
While stretching of most polymer chains leads to rather featureless force-extension diagrams, some, notably DNA, exhibit non-trivial behavior with a distinct plateau region.
A. V. Onufriev, A. V. Savin, Allemand, Bustamante, Bustamante, Case, Cluzel, Garcia, Honig, I. P. Kikot, Li, M. A. Mazo, McCauley, Nelson, Paik, Rief, Savin, Schwaiger, Smith, Storm, Zegarra, Zolotaryuk   +21 more
core   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Helix Folding in One Dimension: Effects of Proline Co-Solvent on Free Energy Landscape of Hydrogen Bond Dynamics in Alanine Peptides

Life
The effects of proline co-solvent on helix folding are explored through the single discrete coordinate of the number of helical hydrogen bonds. The analysis is based on multi-microsecond length molecular dynamics simulations of alanine-based helix ...
Krzysztof Kuczera
doaj   +1 more source

System-Size Dependence of Helix-Bundle Formation for Generic Semiflexible Polymers [PDF]

Polymers, 2016
Helical polymer bundles are an important fixture in biomolecular systems. The particular structural geometry of helix bundles is dependent on many factors including the length of the polymer chain. In this study, we performed Monte Carlo simulations of a coarse-grained model for helical polymers to determine the influence of polymer length on tertiary ...
Williams, Matthew J., Bachmann, Michael
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Electrostatic bending response of a charged helix

, 2017
We explore the electrostatic bending response of a chain of charged particles confined on a finite helical filament. We analyze how the energy difference $\Delta E$ between the bent and the unbent helical chain scales with the length of the helical ...
Schmelcher, P., Stockhofe, J., Zampetaki, A. V.   +2 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

SVM based model generation for binding site prediction on helix turn helix motif type of transcription factors in eukaryotes [PDF]

Bioinformation, 2013
Support vector machine is a class of machine learning algorithms which uses a set of related supervised learning methods for classification and regression. Nowadays this method is vividly applied to many detection problems related with secondary structure, tumor cell and binding residue prediction. In this work, support vector machines (SVMs) have been
Mukherjee, Koel, Abhipriya,, Vidyarthi, Ambarish Saran, Pandey, Dev Mani   +3 more
openaire   +2 more sources

Functional Connectivity Linked to Cognitive Recovery After Minor Stroke

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Patients with minor stroke exhibit slowed processing speed and generalized alterations in functional connectivity involving frontoparietal cortex (FPC). The pattern of connectivity evolves over time. In this study, we examine the relationship of functional connectivity patterns to cognitive performance, to determine ...
Vrishab Commuri, Isabella Dallasta, Ciaran Stone, Sophia Girgenti, Neda Gould, Rafael H. Llinas, Jonathan Z. Simon, Elisabeth Breese Marsh   +7 more
wiley   +1 more source

Generation of Structurally and Functionally Distinct Factors from the Basic Helix-Loop-Helix Gene Hes3 by Alternative First Exons [PDF]

Journal of Biological Chemistry, 2000
The basic helix-loop-helix gene Hes3 is expressed by differentiating and mature Purkinje cells in the cerebellum and by neural precursor cells in the embryonal nervous system. We here found that the transcript of cerebellar Hes3, designated Hes3a, has a distinct 5'-terminal structure from that of embryonal Hes3, designated Hes3b, and that the two types
H, Hirata, T, Ohtsuka, Y, Bessho, R, Kageyama   +3 more
openaire   +2 more sources