Results 101 to 110 of about 801,477 (317)

Inter-species horizontal transfer resulting in core-genome and niche-adaptive variation within Helicobacter pylori [PDF]

, 2005
Background Horizontal gene transfer is central to evolution in most bacterial species. The detection of exchanged regions is often based upon analysis of compositional characteristics and their comparison to the organism as a whole.
Boonmee, P, Jarvis, SA, Boonmee, Prawit, Jarvis, Stephen A.; id_orcid, Peden, John F., Saunders, Nigel, Saunders, NJ, Peden, JF, Saunders, Nigel J., Jarvis, Stephen A.   +9 more
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Identification of essential genes by transposon insertion sequencing and genome-scale metabolic model construction in Streptococcus suis

Microbiology Spectrum
Bacterial essential genes are indispensable for the survival of bacteria and therefore are attractive targets for novel anti-microbial drugs. Identifying essential genes provides a roadmap for developing novel antibiotics and anti-microbial therapies. In
Yongqing Zhang, Ruotong Gong, Menglei Liang, Liangsheng Zhang, Xiujian Liu, Jingzi Zeng, Mengli Yan, Dexin Qiu, Rui Zhou, Qi Huang   +9 more
doaj   +1 more source

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205), Ameet J. Pinto (143202), Lutgarde Raskin, Pinto Ameet J., Raskin, L., Ameet J Pinto, Raskin Lutgarde, Pinto, A.   +7 more
core   +1 more source

Gene therapy using bacterial vectors

Frontiers in Bioscience, 2017
Bacteria can be used for gene therapy via two strategies - either by transfection of eukaryotic host cells using bacteria (bactofection) or by alternative gene therapy that does not alter the host genome, but uses the prokaryotic expression system, which can be controlled or stopped from outside.
Peter, Celec, Roman, Gardlik
openaire   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats [PDF]

, 2014
Dealtry S, Ding G-C, Weichelt V, et al. Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats. PLoS ONE.
Thomas Christopher, Carla Martini, Maria, Gaze, William Hugo,, Sipkema Detmer, Sara Sjöling (462200), Smalla, K, Papa, M.F., Del, Smalla, K., Amos, GCA, Sipkema, D, Vincent Dunon, Elizabeth Margaret Helen Wellington (226811), María Florencia Del Papa (526836), Heuer Holger, Andreas Schlüter (201339), Gaze William Hugo, Dunon Vincent, Martini, María Carla,, Dunon, V., Dealtry Simone, Weichelt, V., Heuer, H, Antonio Lagares (526837), Smalla, Kornelia,, Springael, D., Sjöling, Sara,, Schlüter, A, Martini, MC, Antonio Lagares, Schluter, A., Del Papa, M.F., Sjoiling, Sara, Dealtry, S, William Hugo Gaze, Sjöling Sara, Lagares, A, Gaze, W.H., Jan Dirk van Elsas, María Florencia Del Papa, Jan Dirk van Elsas (108387), María Carla Martini (526835), Detmer Sipkema, Elizabeth Margaret Helen Wellington, Weichelt Viola, Dealtry, Simone, Dirk Springael, Elsas, J.D., Wellington, EMH, van Elsas Jan Dirk, Papa, María Florencia Del,, van Elsas, JD, Springael, Dirk, Smalla Kornelia, Ding, G-C, Florencia Del Papa, Maria, Sjoling, S., Dealtry, Simone,, van Elsas, Jan Dirk, Heuer, Holger,, Gaze, William H., Dunon, V, Thomas, Christopher,, Amos Gregory Charles Auton, Springael, Dirk,, Smalla, Kornelia, Elsas, J. D. van, Andreas, Schlüter, Detmer Sipkema (526839), Wellington, E.M.H., Papa, María Florencia Del, Weichelt, Viola, Ding Guo-Chun, Ding, Guo-Chun,, Sjöling, S, Christopher Thomas (502803), Wellington, E. M. H., Heuer, H., Del Papa, MF, Sjoling, Sara, Gregory Charles Auton Amos (526838), Weichelt, Viola,, Wellington, Elizabeth Margaret Helen,, Kornelia Smalla, Ding, G.C., Schlüter, Andreas, Dunon, Vincent,, Simone Dealtry (526833), Sipkema, D., Weichelt, V, Viola Weichelt (526834), Holger Heuer, Wellington, Elizabeth Margaret Helen, Thomas, Christopher M., Thomas, C., Thomas, Christopher, Sara Sjöling, Guo-Chun Ding (392270), Andreas Schlüter, Schlüter, Andreas ; https://orcid.org/, Heuer, Holger, Elsas, Jan Dirk van, Ding, Guo Chun, Schlüter Andreas, Martini María Carla, Dunon, Vincent, Dirk Springael (526840), Springael Dirk, Sipkema, Detmer,, Martini, M.C., Guo-Chun Ding, Amos, Gregory C. A., María Carla Martini, William Hugo Gaze (226795), Ding, Guo-Chun, Amos, Gregory Charles Auton,, Sipkema, Detmer, Martini, María Carla, van Elsas, Jan Dirk,, Vincent Dunon (179030), Schlüter, Andreas,, Lagares, A., Viola Weichelt, Kornelia Smalla (130483), Lagares Antonio, Wellington Elizabeth Margaret Helen, Auton Amos, Gregory Charles, Amos, G.C.A., Lagares, Antonio,, Holger Heuer (392271), Gaze, William Hugo, Dealtry, S., Thomas, Christopher; id_orcid, Springael, D, Papa María Florencia Del, Amos, Gregory Charles Auton, Thomas, C, Del Papa, María Florencia, Gaze, WH, Gregory Charles Auton Amos, Christopher Thomas, Simone Dealtry, Lagares, Antonio   +141 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Transcriptome sequencing and expression analysis in peanut reveal the potential mechanism response to Ralstonia solanacearum infection

BMC Plant Biology
Background Bacterial wilt caused by Ralstonia solanacearum severely affects peanut (Arachis hypogaea L.) yields. The breeding of resistant cultivars is an efficient means of controlling plant diseases.
Xiao Wang, Feiyan Qi, Ziqi Sun, Hongfei Liu, Yue Wu, Xiaohui Wu, Jing Xu, Hua Liu, Li Qin, Zhenyu Wang, Suling Sang, Wenzhao Dong, Bingyan Huang, Zheng Zheng, Xinyou Zhang   +14 more
doaj   +1 more source