Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan +23 more
core +1 more source
The complete mitochondrial DNA sequence of Putian Loquat longicorn (Anoplophora chinensis)
The complete mitochondrial genome of Putian Loquat longicorn (Anoplophora chinensis) was determined in this study. Its mitochondrial genome (15,871 bp) contains 13 protein-coding genes, 22 tRNA genes, two ribosomal RNA genes (12S rRNA and 16S rRNA), and ...
Long-Wei Fu +6 more
doaj +1 more source
A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]
Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Kowalchuk, GA +30 more
core +1 more source
Evolutionary dynamics of rRNA gene clusters in cichlid fish
Background Among multigene families, ribosomal RNA (rRNA) genes are the most frequently studied and have been explored as cytogenetic markers to study the evolutionary history of karyotypes among animals and plants. In this report, we applied cytogenetic
Nakajima Rafael T +4 more
doaj +1 more source
Epigenetic silencing of nucleolar rRNA genes in Alzheimer's disease. [PDF]
Ribosomal deficits are documented in mild cognitive impairment (MCI), which often represents an early stage Alzheimer's disease (AD), as well as in advanced AD. The nucleolar rRNA genes (rDNA), transcription of which is critical for ribosomal biogenesis,
Maciej Pietrzak +4 more
doaj +1 more source
Compilation of 5S rRNA and 5S rRNA gene sequences
The BERLIN RNA DATABANK as of December 31, 1987, contains a total of 509 sequences of 5S rRNAs or their genes, which is an increase of 45% over the last (1986) compilation (1). It covers sequences from 38 archaebacteria, 184 eubacteria, 14 plastids, 4 mitochondria, 258 eukaryotes and 11 eukaryotic pseudogenes. The BERLIN RNA DATABANK uses the format of
J, Wolters, V A, Erdmann
openaire +3 more sources
Transposition of a chromosomal segment bounded by redundant rRNA genes into other rRNA genes in Escherichia coli [PDF]
We have constructed several mutants of Escherichia coli which have the chromosomal segment between the directly repeated rrnB and rrnE genes deleted from the normal position and transposed into another one of the seven redundant rRNA genes. We have examples where the transposition has been into rrnC, rrnD, rrnG, and rrnH.
C W, Hill, B W, Harnish
openaire +2 more sources
Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences [PDF]
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, Arjen +8 more
core +1 more source
Genetic features of the marine polychaete Sirsoe methanicola from metagenomic data
The methane ice worm Sirsoe methanicola is the only marine polychaete species observed to colonize the methane hydrates of the Gulf of Mexico. Methane hydrates are ephemeral features of deep-sea cold seeps, and finding worm-colonized hydrates is rare ...
Shen Jean Lim +3 more
doaj +1 more source
Molecular cloning of the rRNA genes of Prevotella bryantii B14
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj +1 more source

