Results 81 to 90 of about 5,662,228 (148)

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Proceedings of the National Academy of Sciences of the United States of America, 2000
K. Datsenko, B. Wanner
semanticscholar   +1 more source

Massively parallel reporter assay confirms regulatory potential of hQTLs and reveals important variants in lupus and other autoimmune diseases

HGG Advances
Summary: We designed a massively parallel reporter assay (MPRA) in an Epstein-Barr virus transformed B cell line to directly characterize the potential for histone post-translational modifications, i.e., histone quantitative trait loci (hQTLs ...
Yao Fu, Jennifer A. Kelly, Jaanam Gopalakrishnan, Richard C. Pelikan, Kandice L. Tessneer, Satish Pasula, Kiely Grundahl, David A. Murphy, Patrick M. Gaffney   +8 more
doaj   +1 more source

tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.

Nucleic Acids Research, 1997
T. Lowe, S. Eddy
semanticscholar   +1 more source

Rationally Designed TadA‐Derived Cytosine Editors Enable Context‐Independent Zebrafish Genome Editing

Advanced Science
CRISPR base editors are crucial for precise genome manipulation. Existing APOBEC‐based cytosine base editors (CBEs), while powerful, exhibit indels and sequence context limitations, and editing CC and GC motifs is challenging and inefficient.
Wei Qin, Sheng‐Jia Lin, Yu Zhang, Kevin Huang, Cassidy Petree, Kevin Boyd, Pratishtha Varshney, Gaurav K. Varshney   +7 more
doaj   +1 more source

Epigenetics of prenatal stress in humans: the current research landscape

Clinical Epigenetics
Fetal exposure to prenatal stress can have significant consequences on short- and long-term health. Epigenetic mechanisms, especially DNA methylation (DNAm), are a possible process how these adverse environmental events could be biologically embedded. We
Linda Dieckmann, Darina Czamara
doaj   +1 more source

Neurexin facilitates glycosylation of Dystroglycan to sustain muscle architecture and function in Drosophila

Communications Biology
Neurexin, a molecule associated with autism spectrum disorders, is thought to function mainly in neurons. Recently, it was reported that Neurexin is also present in muscle, but the role of Neurexin in muscle is still poorly understood.
Yu Zhao, Junhua Geng, Zhu Meng, Yichen Sun, Mengzhu Ou, Lizhong Xu, Moyi Li, Guangming Gan, Menglong Rui, Junhai Han, Wei Xie   +10 more
doaj   +1 more source

Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization.

Molecular Biology of the Cell, 1998
P. Spellman, G. Sherlock, Michael Ruogu Zhang, V. Iyer, Klaus E. Anders, M. Eisen, P. Brown, D. Botstein, B. Futcher   +8 more
semanticscholar   +1 more source

ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish

Nature Communications
Advancements in CRISPR technology, particularly the development of base editors, revolutionize genetic variant research. When combined with model organisms like zebrafish, base editors significantly accelerate and refine in vivo analysis of genetic ...
Wei Qin, Fang Liang, Sheng-Jia Lin, Cassidy Petree, Kevin Huang, Yu Zhang, Lin Li, Pratishtha Varshney, Philippe Mourrain, Yanmei Liu, Gaurav K. Varshney   +10 more
doaj   +1 more source

Master transcription factors and mediator establish super-enhancers at key cell identity genes.

Cell, 2013
Warren A. Whyte, David A. Orlando, Denes Hnisz, B. Abraham, Charles Y. Lin, M. Kagey, P. Rahl, Tong Ihn Lee, R. Young   +8 more
semanticscholar   +1 more source

tRNAscan-SE: Searching for tRNA Genes in Genomic Sequences.

Methods in molecular biology, 2019
Patricia P. Chan, T. Lowe
semanticscholar   +1 more source