Results 151 to 160 of about 434,115 (269)

StayRose: A photostable StayGold derivative redshifted by genetic code expansion. [PDF]

J Biol Chem
Scott W, Ivorra-Molla E, Akhuli D, Massam-Wu T, Lysyganicz PK, Walsh R, Parent M, Cook J, Song L, Kumar A, Schneider F, Mishima M, Crow A, Balasubramanian MK.   +13 more
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Corrigendum: Algebraic and toroidal representation of the genetic code

Frontiers in Applied Mathematics and Statistics
Rodrigo Rodríguez-Gutiérrez, Francisco Hernandez-Cabrera, Francisco Javier Almaguer-Martínez, José De Jesús Bernal-Alvarado   +3 more
doaj   +1 more source

<i>Chlamydomonas</i> chloroplast genes tolerate compression of the genetic code to just 51 codons. [PDF]

Proc Natl Acad Sci U S A
Mordaka PM, Clouston K, Cui J, Holzer A, Jackson HO, Purton S, Smith AG.   +6 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Hijacking a bacterial ABC transporter for genetic code expansion. [PDF]

Nature
Iype T, Fottner M, Böhm P, Piedrafita C, Möller Y, Groll M, Lang K.   +6 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Covalent capture and genetic code expansion enables chemoproteomic profiling and functional characterization of lysine acetoacetylation. [PDF]

Sci Adv
Song X, Lu Y, Guo X, Zheng Y, Huang H.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Expanded diversity of pedinophytes provides a window into the evolution of the genetic code in organelles. [PDF]

PLoS Genet
Barcytė D, Žihala D, Eliáš M.
europepmc   +1 more source