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Building biomaterials through genetic code expansion
Trends in Biotechnology, 2023Genetic code expansion (GCE) enables directed incorporation of noncoded amino acids (NCAAs) and unnatural amino acids (UNAAs) into the active core that confers dedicated structure and function to engineered proteins. Many protein biomaterials are tandem repeats that intrinsically include NCAAs generated through post-translational modifications (PTMs ...
Niraikulam Ayyadurai +1 more
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Genetic Code Expansion in Mammalian Cells
2023The expansion of the genetic code has enabled the incorporation of noncanonical amino acids (ncAAs) into a defined site of proteins. By introducing such a unique handle into the protein of interest (POI), bioorthogonal reactions can be utilized in live cells to monitor or manipulate the interaction, translocation, function, and modification of the POI.
Zhigang, Wu, Jie, Wang
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Natural expansion of the genetic code
Nature Chemical Biology, 2006At the time of its discovery four decades ago, the genetic code was viewed as the result of a "frozen accident." Our current knowledge of the translation process and of the detailed structure of its components highlights the roles of RNA structure (in mRNA and tRNA), RNA modification (in tRNA), and aminoacyl-tRNA synthetase diversity in the evolution ...
Alexandre, Ambrogelly +2 more
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2020
The genetic code consists of codons that code for 20 canonical amino acids and 3 termination signals and is an indispensable element of life. It controls all cellular activities through expression of genes. Advances in the genetic engineering techniques have enabled the incorporation of novel non-canonical amino acids (ncAAs) into proteins to confer ...
Nisarg Gohil +2 more
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The genetic code consists of codons that code for 20 canonical amino acids and 3 termination signals and is an indispensable element of life. It controls all cellular activities through expression of genes. Advances in the genetic engineering techniques have enabled the incorporation of novel non-canonical amino acids (ncAAs) into proteins to confer ...
Nisarg Gohil +2 more
openaire +1 more source
The central role of tRNA in genetic code expansion [PDF]
Biochimica Et Biophysica Acta - General Subjects, 2017 The development of orthogonal translation systems (OTSs) for genetic code expansion (GCE) has allowed for the incorporation of a diverse array of non-canonical amino acids (ncAA) into proteins. Transfer RNA, the central molecule in the translation of the genetic message into proteins, plays a significant role in the efficiency of ncAA incorporation ...
Ana Crnkovic +2 more
exaly +3 more sources
An evolved ribosome for genetic code expansion
Nature Biotechnology, 2007Directed evolution of an orthogonal ribosome improves the efficiency of unnatural amino acid incorporation in vivo.
Caroline, Köhrer, Uttam L, RajBhandary
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Genetic Code Expansion: Inception, Development, Commercialization
Journal of the American Chemical Society, 2021Virtually all natural proteins are built from only 20 amino acids, and while this makes possible all the functions they perform, the ability to encode other amino acids selected for specific purposes promises to enable the discovery and production of proteins with novel functions, including therapeutic proteins with more optimal drug-like properties ...
Miglena Manandhar +2 more
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Selenoprotein synthesis: an expansion of the genetic code
Trends in Biochemical Sciences, 1991A number of enzymes employ the unusual amino acid selenocysteine as part of their active site because of its high chemical reactivity. Selenocysteine is incorporated into these proteins co-translationally: biosynthesis occurs on a specific tRNA and insertion into a growing polypeptide is directed by a UGA codon in the mRNA. In E.
A, Böck +3 more
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Nature Structural & Molecular Biology, 2003
Expanding the genetic code by ribosome-mediated introduction of non-natural amino acids into proteins requires engineering of aminoacyl-tRNA synthetase–tRNA pairs. Structural information for pairs specific for tyrosine reveals how this goal may be achieved on a rational basis.
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Expanding the genetic code by ribosome-mediated introduction of non-natural amino acids into proteins requires engineering of aminoacyl-tRNA synthetase–tRNA pairs. Structural information for pairs specific for tyrosine reveals how this goal may be achieved on a rational basis.
openaire +2 more sources
Engineering Translation Components for Genetic Code Expansion
Journal of Molecular Biology, 2022The expansion of the genetic code consisting of four bases and 20 amino acids into diverse building blocks has been an exciting topic in synthetic biology. Many biochemical components are involved in gene expression; therefore, adding a new component to the genetic code requires engineering many other components that interact with it.
Sooin, Kim +3 more
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