Results 241 to 250 of about 273,398 (272)

Natural expansion of the genetic code

Nature Chemical Biology, 2006
At the time of its discovery four decades ago, the genetic code was viewed as the result of a "frozen accident." Our current knowledge of the translation process and of the detailed structure of its components highlights the roles of RNA structure (in mRNA and tRNA), RNA modification (in tRNA), and aminoacyl-tRNA synthetase diversity in the evolution ...
Alexandre, Ambrogelly, Sotiria, Palioura, Dieter, Söll   +2 more
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Genetic code expansion

Nature Structural & Molecular Biology, 2003
Expanding the genetic code by ribosome-mediated introduction of non-natural amino acids into proteins requires engineering of aminoacyl-tRNA synthetase–tRNA pairs. Structural information for pairs specific for tyrosine reveals how this goal may be achieved on a rational basis.
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An evolved ribosome for genetic code expansion

Nature Biotechnology, 2007
Directed evolution of an orthogonal ribosome improves the efficiency of unnatural amino acid incorporation in vivo.
Caroline, Köhrer, Uttam L, RajBhandary
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Expansion of the Genetic Code

2020
The genetic code consists of codons that code for 20 canonical amino acids and 3 termination signals and is an indispensable element of life. It controls all cellular activities through expression of genes. Advances in the genetic engineering techniques have enabled the incorporation of novel non-canonical amino acids (ncAAs) into proteins to confer ...
Nisarg Gohil, Gargi Bhattacharjee, Vijai Singh   +2 more
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Trapping Transient Protein Species by Genetic Code Expansion

ChemBioChem, 2020
AbstractNature employs a limited number of genetically encoded amino acids for the construction of functional proteins. By engineering components of the cellular translation machinery, however, it is now possible to genetically encode noncanonical building blocks with tailored electronic and structural properties.
Matthias Tinzl, Donald Hilvert
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Genetic Code Expansion in Enteric Bacterial Pathogens

2018
The genetic code expansion strategy has become an elegant method for site-specific incorporation of noncanonical amino acids with diverse functionalities into proteins of interest in bacteria, yeast, mammalian cells, and even animals. This technique allows precise labeling as well as manipulation of a given protein to dissect its physiological and/or ...
Huangtao, Zheng, Shixian, Lin, Peng R, Chen   +2 more
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Selenoprotein synthesis: an expansion of the genetic code

Trends in Biochemical Sciences, 1991
A number of enzymes employ the unusual amino acid selenocysteine as part of their active site because of its high chemical reactivity. Selenocysteine is incorporated into these proteins co-translationally: biosynthesis occurs on a specific tRNA and insertion into a growing polypeptide is directed by a UGA codon in the mRNA. In E.
A, Böck, K, Forchhammer, J, Heider, C, Baron   +3 more
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Genetic code expansion for ageing study

2020
Halogen modified Tyr analogs (HMYs) are typical products of the Tyr residue oxidation in proteins. Their occurrence may lead to destabilized protein integrity and functions, ultimately contributing to ageing processes[1]. In this study, I investigated the effects of the HMYs on model proteins.
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Genetic Code Expansion in Pseudomonas putida KT2440

Emerging microorganism Pseudomonas putida KT2440 is utilized for the synthesis of biobased chemicals from renewable feedstocks and for bioremediation. However, the methods for analyzing, engineering, and regulating the biosynthetic enzymes and protein complexes in this organism remain underdeveloped.Such attempts can be advanced by the genetic code ...
Tianyu, Gao, Jiantao, Guo, Wei, Niu
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Efficient Genetic Code Expansion Without Host Genome Modifications

Nature Biotechnology
AbstractSupplementing translation with non-canonical amino acids (ncAAs) can yield protein sequences with new-to-nature functions, but existing ncAA incorporation strategies suffer from low efficiency and context dependence. We uncover codon usage as a previously unrecognized contributor to efficient genetic code expansion using non-native codons ...
Alan Costello, Alexander A. Peterson, David L. Lanster, Zhiyi Li, Gavriela D. Carver, Ahmed H. Badran   +5 more
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