Results 61 to 70 of about 4,608,949 (263)
Genetic Code Expansion and Bio-Orthogonal Labeling Reveal Intact HIV-1 Capsids inside the Nucleus
mBio, 2022 Microscopy is one of the few techniques that can directly observe the HIV-1 capsid as it traverses the cell. However, an extrinsic or intrinsic label is needed to facilitate detection and this can perturb capsid behavior. Now, S. Schifferdecker, V. Zila,
Leo C. James
doaj +1 more source
Therapeutic Apheresis and Dialysis, EarlyView.ABSTRACT Introduction Peritoneal dialysis (PD) is an established home‐based kidney replacement therapy (KRT), but its uptake remains low in Japan. We evaluated whether individualized education in a dedicated outpatient clinic was associated with the initiation of PD.
Yasuko Ito +7 more
wiley +1 more source
Photocrosslinking-induced CRAC channel-like Orai1 activation independent of STIM1
Nature Communications, 2023 The Ca2+ ion channel Orai1 is crucial in immune cells. Here, the authors applied genetic code expansion to transfer light-sensitivity to the Orai1 channel and achieved precise control over its function.
Lena Maltan +16 more
doaj +1 more source
Convenient Genetic Encoding of Phenylalanine Derivatives through Their α-Keto Acid Precursors
Biomolecules, 2021 The activity and function of proteins can be improved by incorporation of non-canonical amino acids (ncAAs). To avoid the tedious synthesis of a large number of chiral phenylalanine derivatives, we synthesized the corresponding phenylpyruvic acid ...
Li Liu +8 more
doaj +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Engineering the tryptophanyl tRNA synthetase and tRNATRP for the orthogonal expansion of the genetic code [PDF]
, 2008 textOver the last twenty years, the expansion of the genetic code has been made possible by the encoding of unnatural amino acids into proteins. Unnatural amino acids could be used to expand the chemical functionalities available to biology allowing for ...
Hughes, Randall Allen, 1978-
core
Transcriptomics of HEK293T and Vero cells influenced by genetic code expansion
, 2022 Transcriptomics of HEK293T and Vero and their derived cells. The derived stable cell lines were engineered with a genetic code expansion (GCE) system. The unnatural amino acid, NAEK, were added to test the effects of genetic code expansion on these cell ...
Haoran Zhang (12577111)
core +1 more source
Genetic code expansion for multiprotein complex engineering [PDF]
Nature Methods, 2016 We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins ...
Christine Koehler +26 more
openaire +4 more sources
Simplified methodology for a modular and genetically expanded protein synthesis in cell-free systems
Synthetic and Systems Biotechnology, 2019 Genetic code expansion, which enables the site-specific incorporation of unnatural amino acids into proteins, has emerged as a new and powerful tool for protein engineering.
Yonatan Chemla +5 more
doaj +1 more source
Frontiers in Synaptic Neuroscience, 2021 Fluorescence labeling of difficult to access protein sites, e.g., in confined compartments, requires small fluorescent labels that can be covalently tethered at well-defined positions with high efficiency.
Alexander Kuhlemann +12 more
doaj +1 more source