Results 71 to 80 of about 733,956 (256)
Genetic monitoring tools for radon pollution research [PDF]
BIO Web of ConferencesA critical target for ionizing radiation is DNA that damage to a cell. Thus, when dealing with the general irradiation of the body, we observe the diverse effects of radiation.
Biyasheva Zarema +3 more
doaj +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Genetic modification of Streptococcus dysgalactiae by natural transformation
mSphereStreptococcus dysgalactiae is an emerging human and animal pathogen. Functional studies of genes involved in virulence of S. dysgalactiae and other pyogenic group streptococci are often hampered by limited genetic tractability.
Marita Torrissen Mårli +4 more
doaj +1 more source
Computational Tools to Investigate Genetic Cardiac Channelopathies
Frontiers in Physiology, 2013 NA
Hugues eAbriel +4 more
doaj +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
On the Choice of the Right Plasmid Vector(s) in the Times of Synthetic Biology
Microbial BiotechnologyPlasmid vectors are to this day the fundamental tools in molecular biology, but their selection is often guided by convenience rather than informed choice. This article revisits the architectural and functional features that determine plasmid performance
Víctor deLorenzo +1 more
doaj +1 more source
Frontiers in MicrobiologyIn eukaryotes and bacteria, it is well-established that the genomic location of ectopic gene integration influences the expression level due to replication-associated gene dosage effects as well as effects mediated by chromatin organization. In contrast,
Yifei Xu +5 more
doaj +1 more source
Assessing Plant Genetic Diversity by Molecular Tools
Diversity, 2009 This paper is an overview of the diverse, predominantly molecular techniques, used in assessing plant genetic diversity. In recent years, there has been a significant increase in the application of molecular genetic methods for assessing the conservation
Linda Mondini +2 more
doaj +1 more source