Results 61 to 70 of about 617,752 (328)

Developing Universal Genetic Tools for Rapid and Efficient Deletion Mutation in Vibrio Species Based on Suicide T-Vectors Carrying a Novel Counterselectable Marker, vmi480. [PDF]

PLoS ONE, 2015
Despite that Vibrio spp. have a significant impact on the health of humans and aquatic animals, the molecular basis of their pathogenesis is little known, mainly due to the limited genetic tools for the functional research of genes in Vibrio.
Peng Luo, Xiangyan He, Qiuting Liu, Chaoqun Hu   +3 more
doaj   +1 more source

Applications and developments of gene therapy drug delivery systems for genetic diseases

Asian Journal of Pharmaceutical Sciences, 2021
Genetic diseases seriously threaten human health and have always been one of the refractory conditions facing humanity. Currently, gene therapy drugs such as siRNA, shRNA, antisense oligonucleotide, CRISPR/Cas9 system, plasmid DNA and miRNA have shown ...
Xiuhua Pan, Hanitrarimalala Veroniaina, Nan Su, Kang Sha, Fenglin Jiang, Zhenghong Wu, Xiaole Qi   +6 more
doaj   +1 more source

De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications. [PDF]

, 2015
Mammalian artificial chromosomes (MACs) are non-integrating, autonomously replicating natural chromosome-based vectors that may carry a vast amount of genetic material, which in turn enable potentially prolonged, safe, and regulated therapeutic transgene
Katona, Róbert László
core   +1 more source

An Efficient Vector System to Modify Cells Genetically

PLoS ONE, 2011
The transfer of foreign genes into mammalian cells has been essential for understanding the functions of genes and mechanisms of genetic diseases, for the production of coding proteins and for gene therapy applications. Currently, the identification and selection of cells that have received transferred genetic material can be accomplished by methods ...
Huamin Han, Wen He, Qingjun Liu, Kristy Ong, Xiaoli Liu, Bin Gao   +5 more
openaire   +5 more sources

Structural dynamics of the plant hormone receptor ETR1 in a native‐like membrane environment

FEBS Letters, EarlyView.
The present study unveils the structural and signaling dynamics of ETR1, a key plant ethylene receptor. Using an optimized nanodisc system and solution NMR, we captured full‐length ETR1 in a native‐like membrane environment. Our findings reveal dynamic domain uncoupling and Cu(I)‐induced rigidification, providing the first evidence of metal‐triggered ...
Moritz Lemke, Nils Alexander Lakomek, Georg Groth   +2 more
wiley   +1 more source

Efficient methods of automatic calibration for rainfall-runoff modelling in the Floreon+ system [PDF]

, 2017
Calibration of rainfall-runoff model parameters is an inseparable part of hydrological simulations. To achieve more accurate results of these simulations, it is necessary to implement an efficient calibration method that provides sufficient refinement of
Kuchař, Štěpán, Portero, Antonio, Theuer, Matyáš, Vavřík, Radim, Vondrák, Vít, Šír, Boris   +5 more
core   +1 more source

Mechanistic basis for inhibition of the extended‐spectrum β‐lactamase GES‐1 by enmetazobactam and tazobactam

FEBS Letters, EarlyView.
Antimicrobial resistance (AMR) is of huge importance, resulting in over 1 million deaths each year. Here, we describe how a new drug, enmetazobactam, designed to help fight resistant bacterial diseases, inhibits a key enzyme (GES‐1) responsible for AMR. Our data show it is a more potent inhibitor than the related tazobactam, with high‐level computation
Michael Beer, Philip Hinchliffe, Marko Hanževački, Christopher R. Bethel, Catherine L. Tooke, Marc W. Van der Kamp, Krisztina M. Papp‐Wallace, Robert A. Bonomo, Stuart Shapiro, Adrian J. Mulholland, James Spencer   +10 more
wiley   +1 more source

Intracranial hemorrhage detection in 3D computed tomography images using a bi-directional long short-term memory network-based modified genetic algorithm

Frontiers in Neuroscience, 2023
IntroductionIntracranial hemorrhage detection in 3D Computed Tomography (CT) brain images has gained more attention in the research community. The major issue to deal with the 3D CT brain images is scarce and hard to obtain the labelled data with better ...
Jewel Sengupta, Robertas Alzbutas, Przemysław Falkowski-Gilski, Bożena Falkowska-Gilska   +3 more
doaj   +1 more source

Frontiers of parasitology research in the People's Republic of China : infection, diagnosis, protection and surveillance [PDF]

, 2012
: Control and eventual elimination of human parasitic diseases in the People's Republic of China (P.R. China) requires novel approaches, particularly in the areas of diagnostics, mathematical modelling, monitoring, evaluation, surveillance and public ...
Bergquist, R., Chen, J. H., Chen, J. X., Tanner, M., Utzinger, J., Wang, H., Zhou, X. N.   +6 more
core   +2 more sources

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

FEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican, Yara Nabawi, William Hongyu Zhang, Dunja Petrovic, Markus Wehrmann, Sara Muñoz‐García, Seda Koyuncu, David Vilchez   +7 more
wiley   +1 more source