Results 171 to 180 of about 43,083,361 (310)

The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection

Molecular Oncology, EarlyView.
Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński, Krzysztof Pastuszak, Anna Samelak‐Czajka, Zuzanna Cemka, Paulina Jackowiak, Tomasz Stefaniak, Sylwia Łapińska‐Szumczyk, Jacek Jassem, Anna J. Żaczek, Matthew T. Rondina, Myron G. Best, Sjors G. J. G. In 't Veld, Anna Supernat   +12 more
wiley   +1 more source

Overcoming platinum resistance by the disulfiram metabolite CuET in novel preclinical models of human carcinomas

Molecular Oncology, EarlyView.
Cisplatin‐resistant cancer cells survive DNA‐damaging platinum‐based treatment yet remain sensitive to CuET‐induced proteotoxic stress. The distinct mechanism of action allows CuET to overcome and eliminate cisplatin‐resistant tumour cells, unless highly expressed metallothioneins (MTs) interfere with CuET, thereby highlighting MT levels as candidate ...
Stefanos Arampatzis, Tereza Buchtová, Jirina Bartkova, Marie Lund Bay, Christoffer Lynge Halvorsen, Zuzana Machacova, Zdenek Skrott, Martin Mistrik, Ekaterina Dulina, Miguel Andujar‐Sanchez, Estrid Høgdall, Tine Henrichsen Schnack, Claus Høgdall, Berit Jul Mosgaard, Marie Boennelycke, Jacob Brink Laursen, Allan Jensen, Susanne K. Kjær, Tuula Kallunki, Robert Strauss, Jiri Bartek   +20 more
wiley   +1 more source

An outer membrane vesicle‐associated two‐track model for enterotoxigenic Bacteroides fragilis oncogenesis

Molecular Oncology, EarlyView.
Enterotoxigenic Bacteroides fragilis is a gut bacterium linked to bowel cancer. We propose that its toxin acts along two routes: at the cell surface, cutting the adhesion protein E‐cadherin and triggering inflammation; and inside the cell, delivered by bacterial vesicles, switching on NOD1 and cancer stem cell programs.
Kangchan Choi, Sang‐Hyeon Yoo, Ki‐Jong Rhee   +2 more
wiley   +1 more source

A genome-wide association study of intra-ocular pressure suggests a novel association in the gene FAM125B in the TwinsUK cohort

, 2014
Glaucoma is a major cause of blindness in the world. To date, common genetic variants associated with glaucoma only explain a small proportion of its heritability. We performed a genome-wide association study of intra-ocular pressure (IOP), an underlying
Aung, T., van Koolwijk, L.M., Tanja, Z., Khor, C.-C., Venturini, C., Mackey, D.A., Jonas, J.B., Hammond, C., Vitart, V., Viswanathan, A.C., Hewitt, A.W., Gibson, J., Klaver, C.C., Nag, A., WTCCC, None, Xu, L., Lemij, H.G., Liao, J., Wang, J.J., Mitchell, P., Young, T. L., Macgregor, S., Ramdas, W.D., Vithana, E., Hofman, A., Lu, Y., Small, K.S., Wojciechowski, R., van Duijn, C.M., Hysi, P.G., Springelkamp, H., Yonova-Doing, E., Fleck, B.W., Williams, K.M., Wong, T.-Y., Uitterlinden, A.G., Lotery, A.J., Cheng, C.-Y., Tai, E.-S., Hohn, R., Teo, Y.-Y., Vingerling, J.R., Young, Terri L, Nag, Abhishek, Mackey, David A, Small, Kerrin S; id_orcid, ?, ?, Hammond, Christopher J, Viswanathan, Ananth C, Venturini, Cristina, Hysi, Pirro G   +50 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Admixed and single-continental genome segments of the same ancestry have distinct linkage disequilibrium patterns

Genome Biology
Background Admixed populations offer valuable insight into the genetic architecture of complex traits. Many studies have proposed methods for genome-wide association study (GWAS) in admixed populations and various simulation studies have evaluated their ...
Hanbin Lee, Moo Hyuk Lee, Kangcheng Hou, Bogdan Pasaniuc, Buhm Han   +4 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Deciphering the Genetic Basis of Allelopathy in japonica Rice Cultivated in Temperate Regions Using a Genome-Wide Association Study

Rice
Allelopathy has been considered as a natural method of weed control. Despite the nature of allelochemical compounds has been studied, little is known about the genetic basis underlying allelopathy.
Julia García-Romeral, Raúl Castanera, Josep Casacuberta, Concha Domingo   +3 more
doaj   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source