Results 131 to 140 of about 2,481,819 (312)

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Assisted assembly: how to improve a de novo genome assembly by using related species [PDF]

, 2009
We describe a new assembly algorithm, where a genome assembly with low sequence coverage, either throughout the genome or locally, due to cloning bias, is considerably improved through an assisting process via a related genome.
Gnerre, Sante, Jaffe, David B., Sante Gnerre, Kerstin Lindblad-Toh, Lander, Eric Steven, Lander, Eric, David B Jaffe, Lander, Eric S, Lindblad-Toh, Kerstin, Eric S Lander, Jaffe, David B, Lindblad-Toh, Kerstin,   +11 more
core   +1 more source

A Preprocessor for Shotgun Assembly of Large Genomes

Journal of Computational Biology, 2004
The whole-genome shotgun (WGS) assembly technique has been remarkably successful in efforts to determine the sequence of bases that make up a genome. WGS assembly begins with a large collection of short fragments that have been selected at random from a genome.
Michael Roberts, Brian R. Hunt, James A. Yorke, Randall A. Bolanos, Arthur L. Delcher   +4 more
openaire   +2 more sources

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Trypanosoma brucei EATRO1125 draft genome assembly (Contigs)

, 2021
Trypanosoma brucei ssp, unicellular parasites causing human and animal trypanosomiasis, are transmitted between mammals by tsetse flies. Periodic changes in variant surface glycoproteins (VSG), which form the parasite coat in the mammal, allow them to ...
Alves Fernandes, Paula, Bevkal Subramanyaswamy, Shubha, Naguleswaran, Arunasalam, Nicholson, Pamela, Roditi, Isabel, Rehmann, Ruth   +5 more
core  

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

The genome sequence of common box, Buxus sempervirens L. (Buxaceae) [version 2; peer review: 2 approved]

Wellcome Open Research
We present a genome assembly from an individual Buxus sempervirens (common box; Streptophyta; Magnoliopsida; Buxales; Buxaceae). The genome sequence has a total length of 676.70 megabases.
Maarten J. M. Christenhusz, Michael F. Fay   +1 more
doaj   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

The genome sequence of the Roundleaf geranium, Geranium rotundifolium L. (Geraniales: Geraniaceae) [version 1; peer review: 2 approved]

Wellcome Open Research
We present a genome assembly of Geranium rotundifolium (Roundleaf geranium; Streptophyta; Magnoliopsida; Geraniales; Geraniaceae). The genome sequence has a total length of 497.00 megabases. Most of the assembly (97.57%) is scaffolded into 13 chromosomal
Maarten J. M. Christenhusz, Alex D. Twyford   +1 more
doaj   +1 more source