Results 11 to 20 of about 526,413 (332)
A Method for Electroporation of Cre Recombinase Protein into Intact Nicotiana tabacum Cells
Plants, 2023 The Cre/lox recombination system has become a powerful technology for gene function analysis in a broad spectrum of cell types and organisms. In our previous report, Cre protein had been successfully delivered into intact Arabidopsis thaliana cells using
Yuichi Furuhata +3 more
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eLife, 2020 Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signaling are implicated in human disease mechanisms.
Deepti Trivedi +8 more
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Tree Genetic Engineering, Genome Editing and Genomics
International Journal of Molecular Sciences, 2022 In this Special Issue [...]
Matthias Fladung, Birgit Kersten
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Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. [PDF]
, 2018 The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus.
Chen, Yuan-Chuan +3 more
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Investigation of Genome Biology by Synthetic Genome Engineering
Bioengineering, 2023 Synthetic genomes were designed based on an understanding of natural genomic information, offering an opportunity to engineer and investigate biological systems on a genome-wide scale. Currently, the designer version of the M.
Hui Zhang, Yao Xiong, Wenhai Xiao, Yi Wu
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Precision genome engineering [PDF]
Current Biology, 2014 What is precision genome engineering? Exactly that! You know what a genome is; ‘engineering’ means that we want to change that DNA, and ‘precision’ means we want to do it in a very specific, targeted way.Sounds challenging, why would you want to do that? Lots of reasons.
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BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]
, 2016 Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien +7 more
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Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule
Frontiers in Plant Science, 2017 The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous delivery of a DNA template for homology-directed repair (HDR).
Haroon Butt +8 more
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Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx [PDF]
, 2017 The genomes of most bacteria contain mobile DNA elements that can contribute to undesirable genetic instability in engineered cells. In particular, transposable insertion sequence (IS) elements can rapidly inactivate genes that are important for a ...
Barrick, Jeffrey E +3 more
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Genome scale engineering techniques for metabolic engineering [PDF]
Metabolic Engineering, 2015 Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in genome-scale engineering technologies. Metabolic engineering has been generally defined by the use of iterative cycles of rational genome modifications, strain analysis and characterization ...
Liu, Rongming +3 more
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