CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]
, 2020 The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I +2 more
core +1 more source
MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells
Viruses, 2019 The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryotic proteins for many applications, ranging from structure analysis to drug screening and the provision of protein biologics and therapeutics.
Kapil Gupta +5 more
doaj +1 more source
Genome engineering of stem cells for autonomously regulated, closed-loop delivery of biologic drugs [PDF]
, 2017 Chronic inflammatory diseases such as arthritis are characterized by dysregulated responses to pro-inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α).
Brunger, Jonathan M +4 more
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Multiplexed CRISPR-Cas9-Based Genome Editing of
mSphere, 2019 Microbial production of biofuels and bioproducts offers a sustainable and economic alternative to petroleum-based fuels and chemicals. The basidiomycete yeast Rhodosporidium toruloides is a promising platform organism for generating bioproducts due to ...
Peter B. Otoupal +5 more
doaj +1 more source
CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering
Molecular Systems Biology, 2020 Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target ...
Daniel Paolo Anderson +3 more
doaj +1 more source
Live pigs produced from genome edited zygotes [PDF]
, 2013 Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome.
AJ Clark +13 more
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Improving single-cell cloning workflow for gene editing in human pluripotent stem cells [PDF]
, 2018 The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens.
Chen, Yi-Hsien, Pruett-Miller, Shondra M
core +2 more sources
Biological Parts for Kluyveromyces marxianus Synthetic Biology
Frontiers in Bioengineering and Biotechnology, 2019 Kluyveromyces marxianus is a non-conventional yeast whose physiology and metabolism lends itself to diverse biotechnological applications. While the wild-type yeast is already in use for producing fragrances and fermented products, the lack of ...
Arun S. Rajkumar +4 more
doaj +1 more source
Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]
, 2020 Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu +2 more
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Genome Editing in Trees: From Multiple Repair Pathways to Long-Term Stability
Frontiers in Plant Science, 2018 The CRISPR technology continues to diversify with a broadening array of applications that touch all kingdoms of life. The simplicity, versatility and species-independent nature of the CRISPR system offers researchers a previously unattainable level of ...
William Patrick Bewg +3 more
doaj +1 more source