Results 41 to 50 of about 526,413 (332)
Yeast Genome Engineering [PDF]
SCIENTIA SINICA Vitae, 2015 酿酒酵母染色体的人工合成突破了真核生物基因组重新设计与合成, 将引发基因组工程研究新的高潮. 本文以酵母基因组工程为例, 对“自上而下”和“自下而上”两种不同策略的基因组工程研究取得的最新进展进行综述, 并展望其发展前景和趋势.
HuanMing YANG +5 more
openaire +1 more source
Accelerated genome engineering through multiplexing [PDF]
WIREs Systems Biology and Medicine, 2015 Throughout the biological sciences, the past 15 years have seen a push toward the analysis and engineering of biological systems at the organism level. Given the complexity of even the simplest organisms, though, to elicit a phenotype of interest often requires genotypic manipulation of several loci.
Zehua, Bao, Ryan E, Cobb, Huimin, Zhao
openaire +2 more sources
Synthetic biology—putting engineering into biology [PDF]
, 2006 Synthetic biology is interpreted as the engineering-driven building of increasingly complex biological entities for novel applications. Encouraged by progress in the design of artificial gene networks, de novo DNA synthesis and protein engineering, we ...
Heinemann, Matthias,, Panke, Sven,
core +1 more source
Toward genomic cell culture engineering [PDF]
Cytotechnology, 2006 Genomic and proteomic based global gene expression profiling has altered the landscape of biological research in the past few years. Its potential impact on cell culture bioprocessing has only begun to emanate, partly due to the lack of genomic sequence information for the most widely used industrial cells, Chinese hamster ovary (CHO) cells ...
Katie F, Wlaschin +2 more
openaire +2 more sources
A Computational Method for the Rate Estimation of Evolutionary Transpositions
, 2015 Genome rearrangements are evolutionary events that shuffle genomic architectures. Most frequent genome rearrangements are reversals, translocations, fusions, and fissions.
J. Ma +8 more
core +1 more source
Developments in the tools and methodologies of synthetic biology. [PDF]
, 2014 Synthetic biology is principally concerned with the rational design and engineering of biologically based parts, devices, or systems. However, biological systems are generally complex and unpredictable, and are therefore, intrinsically difficult to ...
Freemont, P +3 more
core +1 more source
Induction of gametocytogenesis in human malaria parasites: from stress to genome editing
Frontiers in MicrobiologyGametocytogenesis is a crucial process in which malaria parasites transition from their asexual stage to the sexual-stage gametocytes. This transformation enables the parasite to infect and multiply within the Anopheles mosquito, the vector responsible ...
Rishitharan Subramaniam +4 more
doaj +1 more source
CRISPR/Cas9 advances engineering of microbial cell factories [PDF]
, 2016 One of the key drivers for successful metabolic engineering in microbes is the efficacy by which genomes can be edited. As such there are many methods to choose from when aiming to modify genomes, especially those of model organisms like yeast and ...
Jakociunas, Tadas +2 more
core +2 more sources
Pharmaceutics, 2020 DNA delivery is at the forefront of current research efforts in gene therapy and synthetic biology. Viral vectors have traditionally dominated the field; however, nonviral delivery systems are increasingly gaining traction.
Francesco Aulicino +2 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2021 The utility of CRISPR-Cas9 to repair or reverse diseased states that arise from recent genetic mutations in the human genome is now widely appreciated. The use of CRISPR to “design” the outcomes of biology is challenged by both specialized ethicists and ...
Lais de Lima Balico, Eric A. Gaucher
doaj +1 more source