Results 51 to 60 of about 3,929,879 (390)
Genome‐scale engineering for systems and synthetic biology
Molecular Systems Biology, 2013 Genome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre‐defined locations across the genome.
Kevin M Esvelt, Harris H Wang
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iPSCs in NK Cell Manufacturing and NKEV Development
Frontiers in Immunology, 2022 Natural killer (NK) cell immunotherapies for cancer can complement existing T cell therapies while benefiting from advancements already made in the immunotherapy field.
Nicholas Boyd-Gibbins +4 more
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Advances in yeast genome engineering [PDF]
FEMS Yeast Research, 2014 Genome engineering based on homologous recombination has been applied to yeast for many years. However, the growing importance of yeast as a cell factory in metabolic engineering and chassis in synthetic biology demands methods for fast and efficient introduction of multiple targeted changes such as gene knockouts and introduction of multistep ...
Florian, David, Verena, Siewers
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Frontiers in Chemistry, 2014 Incorporating non-standard amino acids (NSAAs) into proteins enables new chemical properties, new structures, and new functions. In recent years, improvements in cell-free protein synthesis (CFPS) systems have opened the way to accurate and efficient ...
Seok Hoon eHong +2 more
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Application of Gene Editing Technology in Resistance Breeding of Livestock
Life, 2022 As a new genetic engineering technology, gene editing can precisely modify the specific gene sequence of the organism’s genome. In the last 10 years, with the rapid development of gene editing technology, zinc-finger nucleases (ZFNs), transcription ...
Sutian Wang +8 more
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Improving single-cell cloning workflow for gene editing in human pluripotent stem cells [PDF]
, 2018 The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens.
Chen, Yi-Hsien, Pruett-Miller, Shondra M
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Yeast Genome Engineering [PDF]
SCIENTIA SINICA Vitae, 2015 酿酒酵母染色体的人工合成突破了真核生物基因组重新设计与合成, 将引发基因组工程研究新的高潮. 本文以酵母基因组工程为例, 对“自上而下”和“自下而上”两种不同策略的基因组工程研究取得的最新进展进行综述, 并展望其发展前景和趋势.
HuanMing YANG +5 more
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Accelerated genome engineering through multiplexing [PDF]
WIREs Systems Biology and Medicine, 2015 Throughout the biological sciences, the past 15 years have seen a push toward the analysis and engineering of biological systems at the organism level. Given the complexity of even the simplest organisms, though, to elicit a phenotype of interest often requires genotypic manipulation of several loci.
Zehua, Bao, Ryan E, Cobb, Huimin, Zhao
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Synthetic biology—putting engineering into biology [PDF]
, 2006 Synthetic biology is interpreted as the engineering-driven building of increasingly complex biological entities for novel applications. Encouraged by progress in the design of artificial gene networks, de novo DNA synthesis and protein engineering, we ...
Heinemann, Matthias,, Panke, Sven,
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Toward genomic cell culture engineering [PDF]
Cytotechnology, 2006 Genomic and proteomic based global gene expression profiling has altered the landscape of biological research in the past few years. Its potential impact on cell culture bioprocessing has only begun to emanate, partly due to the lack of genomic sequence information for the most widely used industrial cells, Chinese hamster ovary (CHO) cells ...
Katie F, Wlaschin +2 more
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