Defining synonymous codon compression schemes by genome recoding [PDF]
Nature, 2016 Synthetic recoding of genomes, to remove targeted sense codons, may facilitate the encoded cellular synthesis of unnatural polymers by orthogonal translation systems.
Kaihang Wang +2 more
exaly +8 more sources
Synonymous genome recoding: a tool to explore microbial biology and new therapeutic strategies [PDF]
Nucleic Acids Research, 2019 Synthetic genome recoding is a new means of generating designed organisms with altered phenotypes. Synonymous mutations introduced into the protein coding region tolerate modifications in DNA or mRNA without modifying the encoded proteins.
MIGUEL Angel Martinez +2 more
exaly +8 more sources
Impact of Synonymous Genome Recoding on the HIV Life Cycle [PDF]
Front Microbiol, 2021 Synonymous mutations within protein coding regions introduce changes in DNA or messenger (m) RNA, without mutating the encoded proteins. Synonymous recoding of virus genomes has facilitated the identification of previously unknown virus biological ...
Franco Cirera, Sandra +3 more
core +11 more sources
Development of safe and highly protective live-attenuated SARS-CoV-2 vaccine candidates by genome recoding [PDF]
Cell Reports, 2021 Safe and effective vaccines are urgently needed to stop the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We construct a series of live attenuated vaccine candidates by large-scale recoding of the SARS-CoV-2 genome and ...
Jakob Trimpert +2 more
exaly +6 more sources
ReScribe: An Unrestrained Tool Combining Multiplex Recombineering and Minimal-PAM ScCas9 for Genome Recoding Pseudomonas putida [PDF]
ACS Synthetic Biology, 2021 Genome recoding enables incorporating new functions into the DNA of microorganisms. By reassigning codons to noncanonical amino acids, the generation of new-to-nature proteins offers countless opportunities for bioproduction and biocontainment in ...
Enrique Asin-Garcia +2 more
exaly +5 more sources
Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA. [PDF]
Nat Commun, 2021 While genome recoding using quadruplet codons to incorporate non-proteinogenic amino acids is attractive for biotechnology and bioengineering purposes, the mechanism through which such codons are translated is poorly understood.
Hou, Ya-Ming +9 more
core +7 more sources
Genome recoding by tRNA modifications [PDF]
Open Biology, 2016 RNA modifications are emerging as an additional regulatory layer on top of the primary RNA sequence. These modifications are particularly enriched in tRNAs where they can regulate not only global protein translation, but also protein translation at the ...
Francesca Tuorto, Frank Lyko
exaly +6 more sources
Attenuation of Human Respiratory Viruses by Synonymous Genome Recoding [PDF]
Frontiers in Immunology, 2019 Using computer algorithms and commercial DNA synthesis, one or more ORFs of a microbial pathogen such as a virus can be recoded and deoptimized by several strategies that may involve the introduction of up to thousands of nucleotide (nt) changes without ...
Cyril LE NouËN +2 more
exaly +7 more sources
Synthetic genome recoding: new genetic codes for new features [PDF]
Current Genetics, 2017 Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years.
Yu Heng Lau +2 more
exaly +6 more sources
Flavivirus genome recoding by codon optimisation confers genetically stable in vivo attenuation in both mice and mosquitoes [PDF]
PLoS Pathogens, 2023 Virus genome recoding is an attenuation method that confers genetically stable attenuation by rewriting a virus genome with numerous silent mutations. Prior flavivirus genome recoding attempts utilised codon deoptimisation approaches.
Regina Ching Hua Lee +2 more
exaly +3 more sources