Results 21 to 30 of about 1,107,385 (165)

Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of E. coli in Drinking Water [PDF]

ACS Synth Biol
Modern genome editing methods permit the flexible modification of organisms at the genome level. However, bacteriophages, despite their small genomes, pose unique challenges due to the need to edit during their infection cycle, then select/screen for the
David R Parker, Andrew P. Sikkema, Ranee K. Anderson, Gregory J. S. Lohman, S. Nugen   +4 more
semanticscholar   +2 more sources

Signal peptides restrict genome evolution and A-to-I RNA editing [PDF]

NAR Genom Bioinform
The genetic information flows from DNA to RNA to protein with high fidelity. While highly essential or conserved genomic sequences are often thought to be compensated by post-transcriptional mechanisms such as alternative splicing (AS) and RNA editing to
Y. Duan, Shuxian Chen, Wanzhi Cai, Wen Song, Hu Li   +4 more
semanticscholar   +2 more sources

A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA[Ser]Sec Gene

Cells, 2019
The translation of selenoprotein mRNAs involves a non-canonical ribosomal event in which an in-frame UGA is recoded as a selenocysteine (Sec) codon instead of being read as a stop codon.
Philippe E. Mangeot, Guillin, Olivia, Ohlmann, Théophile, Caroline Vindry, Théophile Ohlmann, Laurent Chavatte, Mangeot, Philippe, Chavatte, Laurent, Olivia Guillin, Vindry, Caroline   +9 more
core   +2 more sources

Blastocrithidia nonstop mitochondrial genome and its expression are remarkably insulated from nuclear codon reassignment.

Nucleic Acids Research
The canonical stop codons of the nuclear genome of the trypanosomatid Blastocrithidia nonstop are recoded. Here, we investigated the effect of this recoding on the mitochondrial genome and gene expression.
Albanaz, Amanda T S, Afonin, Dmitry A, Gahura, Ondřej, Záhonová, Kristína, Škodová-Sveráková, Ingrid, Opperdoes, Frederik, Myšková, Eva, Zimmer, Sara L, Yurchenko, Vyacheslav, Lukeš, Julius, Gerasimov, Evgeny S, Paris, Zdeněk, Bykova, Anastassia, Horváth, Anton   +13 more
core   +2 more sources

Experimental evolution heals the scars of genome-scale recoding [PDF]

Proceedings of the National Academy of Sciences, 2018
Much of the dramatic plot of Mary Shelley’s Frankenstein resulted from the apparent scars and imperfections of the creature her hero brought to life. Similarly, organisms highly modified by synthetic biologists suffer from scars and imperfect functioning that their creators had not intended. However, as presented in PNAS by Wannier et al.
O. Tenaillon
semanticscholar   +3 more sources

Flexible Genome Recoding Strategies for Exploring Codon Space in the Escherichia Coli Genome

, 2018
The degeneracy of the genetic code allows nucleic acids to encode amino acid identity as well as non-coding information for gene regulation and genome maintenance.
Napolitano, Michael G.
core   +2 more sources

Adaptive evolution of genomically recoded Escherichia coli [PDF]

Proceedings of the National Academy of Sciences, 2018
Significance The construction of an organism with an altered genetic code negatively impacted its fitness. We evolved this organism for ∼1,100 generations in the laboratory to recover fitness and learn what changes would accumulate during evolutionary trajectories toward faster growth rates.
Timothy M. Wannier, Aditya M. Kunjapur, Daniel P. Rice, Michael J. McDonald, Michael M. Desai, George M. Church   +5 more
openaire   +3 more sources

Genomically Recoded Organisms Expand Biological Functions [PDF]

Science, 2013
Changing the Code Easily and efficiently expanding the genetic code could provide tools to genome engineers with broad applications in medicine, energy, agriculture, and environmental safety. Lajoie et al. (p.
Lajoie, Marc J., Rovner, Alexis J., Goodman, Daniel B., Aerni, Hans-Rudolf, Haimovich, Adrian D., Kuznetsov, Gleb, Mercer, Jaron A., Wang, Harris H., Carr, Peter A., Mosberg, Joshua A., Rohland, Nadin, Schultz, Peter G., Jacobson, Joseph M., Rinehart, Jesse, Church, George, Isaacs, Farren J.   +15 more
openaire   +4 more sources

Characterisation of DENV2 genome recoding using DENV2-EGFP.

, 2023
DENV2-EGFP is a dengue reporter virus that expresses EGFP. (a) Genomic maps showing regions of the DENV2-EGFP genome recoded with silent mutations. rcE2-90 and rcE2 clones: partial recoding of 3′ segment of Env protein coding region.
Wei-Xin Chin (843752), Xin Jun Hou (17291254), Zi Yun Teo (17291245), Antson Kiat Yee Tan (17291257), Yue Wan (301736), Thinesshwary Yogarajah (7871879), Justin Jang Hann Chu (7310267), Regina Ching Hua Lee (17291251), Roland G. Huber (109002), Hao Yuin Kong (17291239), Yu Cai (112178), Isabelle Xin Yu Zhu (17291242), Bowen Yi (11579422), Si Xian Ho (10125726), Zhen Qin Aw (11828321), Regina Faruk (17291248)   +15 more
core   +1 more source

Measuring amber initiator tRNA orthogonality in a genomically recoded organism [PDF]

ACS Synthetic Biology, 2019
ABSTRACT Using engineered initiator tRNA for precise control of protein translation within cells has great promise within future orthogonal translation systems to decouple housekeeping protein metabolism from that of engineered genetic systems. Previously, E. coli strain C321.ΔA.
Vincent, Russel M., Wright, Bradley W., Jaschke, Paul R.   +2 more
openaire   +2 more sources