Results 11 to 20 of about 1,107,385 (165)

Genome recoding strategies to improve cellular properties: mechanisms and advances [PDF]

aBIOTECH, 2021
The genetic code, once believed to be universal and immutable, is now known to contain many variations and is not quite universal. The basis for genome recoding strategy is genetic code variation that can be harnessed to improve cellular properties. Thus,
Tanya Singh, S. Yadav, A. Vainstein, Vinay Kumar   +3 more
semanticscholar   +6 more sources

Synthetic genomes unveil the effects of synonymous recoding [PDF]

bioRxiv
Engineering the genetic code of an organism provides the basis for (i) making any organism safely resistant to natural viruses and (ii) preventing genetic information flow into and out of genetically modified organisms while (iii) allowing the ...
Ahn, Jenny, Spencer, Owen, Hajian, Behnoush, Jin, Adeline, Nyerges, Akos, Harmon-Smith, Miranda, Blaby, Ian, Budnik, Bogdan, Yoshikuni, Yasuo, Kintses, Balint, Chen, Dawn, Rudolph, Alexandra, Ayalavarapu, Venkat, Tarver, Angela, Church, George M, Baas-Thomas, Maximilien, Hamilton, Matthew, Wang, Meizhou, Shen, Yue, Chiappino-Pepe, Anush, Yan, Shirui, Liu, Min, Szamel, Monika, Chen, Kangming, Flynn, Regan, Seregi, Viktoria, Li, Zilong, Hu, Fangxiang, Ostrov, Nili, Zheng, Qingmei   +29 more
core   +4 more sources

Rapid genome recoding by iterative recombineering of synthetic DNA [PDF]

bioRxiv, 2017
Genome recoding will provide a deeper understanding of genetics and transform biotechnology. We bypass the reliance of previous genome recoding methods on site-specific enzymes and demonstrate a rapid recombineering based strategy for writing genomes by ...
Yu Heng Lau, Adam Riesselman, Matthew T. Weinstock, Jeffrey C. Way, Pamela A. Silver, Connor A. Horton, Daniel G. Gibson, Finn Stirling, Yujia A. Chan, Elena Schaefer, James Kuo, Michiel A.P. Karrenbelt, David Lips   +12 more
core   +3 more sources

Total synthesis of Escherichia coli with a recoded genome [PDF]

Nature, 2019
Nature uses 64 codons to encode the synthesis of proteins from the genome, and chooses 1 sense codon—out of up to 6 synonyms—to encode each amino acid.
Uttamapinant, Chayasith, Julius Fredens, Václav Beránek, Wolfgang H. Schmied, Chin, Jason W., Daniel de la Torre, Chayasith Uttamapinant, Thomas S. Elliott, Andres Gonzalez Llamazares, Schmied, Wolfgang H., Gonzalez Llamazares, Andres, Wesley E. Robertson, Jason W. Chin, Christova, Yonka, Dunkelmann, Daniel L., Robertson, Wesley E., Wang, Kaihang, de la Torre, Daniel, Funke, Louise F. H., Beránek, Václav, Daniel L. Dunkelmann, Elliott, Thomas S., Louise F. H. Funke, Kaihang Wang, Tiongsun Chia, Chia, Tiongsun, Fredens, Julius, Yonka Christova   +27 more
core   +7 more sources

Gene recoding by synonymous mutations creates promiscuous intragenic transcription initiation in mycobacteria

MBio, 2023
Each genome encodes some codons more frequently than their synonyms (codon usage bias), but codons are also arranged more frequently into specific pairs (codon pair bias).
Lia A Parkin, Mary L Previti, Stephanie Müller   +2 more
exaly   +3 more sources

Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA [PDF]

Nucleic Acids Research, 2017
The ability to rewrite large stretches of genomic DNA enables the creation of new organisms with customized functions. However, few methods currently exist for accumulating such widespread genomic changes in a single organism.
Yu Heng Lau, Adam Riesselman, Jeffrey C Way   +2 more
exaly   +4 more sources

Conserved A-to-I RNA editing with non-conserved recoding expands the candidates of functional editing sites

Fly
Adenosine-to-inosine (A-to-I) RNA editing recodes the genome and confers flexibility for the organisms to adapt to the environment. It is believed that RNA recoding sites are well suited for facilitating adaptive evolution by increasing the proteomic ...
Yuange Duan, Fan Song, Wanzhi Cai
exaly   +3 more sources

HIV-1 Protease Evolvability Is Affected by Synonymous Nucleotide Recoding

Journal of Virology, 2018
Large-scale synonymous recoding of virus genomes is a new tool for exploring various aspects of virus biology. Synonymous virus genome recoding can be used to investigate how a virus's position in sequence space defines its mutant spectrum, evolutionary ...
Maria Nevot, Ana Jordan-Paiz, Gloria Martrus   +2 more
exaly   +4 more sources

Genome-wide analysis of differential RNA editing in epilepsy [PDF]

Genome Research, 2017
The recoding of genetic information through RNA editing contributes to proteomic diversity, but the extent and significance of RNA editing in disease is poorly understood.
Michael R. Johnson, Van Eyll, J, Ko, Jeong-Hun, Shkura, Kirill, Sarah R. Langley, Kaminski, RM, KO, J-H, Mazzuferi, Manuela, Ko, J.-H, Langley, Sarah R., Foerch, P, Danis, Bénédicte, Delahaye-Duriez, Andree, Srivastava, P.K, Kaminski, Rafal M., Behmoaras, Jacques, Jeong-Hun Ko, Rotival, M, Enrico Petretto, Andree Delahaye-Duriez, Danis, B, Rafal M. Kaminski, Kumar Srivastava, Prashant, Johnson, Michael R., Langley, S.R, Maxime Rotival, Johnson, M.R, Bagnati, Marta, Eyll, JV, Foerch, Patrik, Srivastava, PK, Delahaye-Duriez, A, Patrik Foerch, Jacques Behmoaras, Kaminski, R.M, Langley, SR, Johnson, MR, Behmoaras, J, Jonathan van Eyll, Petretto, E, Prashant Kumar Srivastava, Mazzuferi, M, van Eyll, Jonathan, Marta Bagnati, Kirill Shkura, Bénédicte Danis, Bagnati, M, Shkura, K, Rotival, Maxime, Petretto, Enrico, Manuela Mazzuferi   +50 more
core   +2 more sources

Applications of recombineering and recoded genome construction.

, 2022
Genome engineering offers the opportunity to bypass natural selection to generate novel organisms. Although multiple types of genome engineering are currently possible, work described in this dissertation occurred in the context of developing a recoded ...
Rudolph, Alexandra Isla
core   +2 more sources