Results 41 to 50 of about 1,107,385 (165)
, 2013 2A oligopeptide sequences (“2As”) mediate a cotranslational recoding event termed “ribosome skipping.” Previously we demonstrated the activity of 2As (and “2A-like sequences”) within a wide range of animal RNA virus genomes and non-long terminal repeat ...
Sukhodub, Andriy +17 more
core +1 more source
Multiplex base editing to convert TAG into TAA codons in the human genome
Nature Communications, 2021 Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 ...
Yuting Chen +7 more
semanticscholar +1 more source
Genomic Recoding Broadly Obstructs the Propagation of Horizontally Transferred Genetic Elements [PDF]
Cell Systems, 2016 Horizontally transferred genetic elements such as viruses and conjugative plasmids move DNA between organisms, increasing genetic diversity but destabilizing engineered biological systems. Here, we used a genomically recoded Escherichia coli strain lacking UAG stop codons and the recognition protein release factor 1 to study how an alternative genetic ...
Natalie Jing, Ma, Farren J, Isaacs
openaire +2 more sources
Frontiers in Microbiology, 2021 Organic Lake in Antarctica is a marine-derived, cold (−13∘C), stratified (oxic-anoxic), hypersaline (>200 gl–1) system with unusual chemistry (very high levels of dimethylsulfide) that supports the growth of phylogenetically and metabolically diverse ...
T. Williams +7 more
semanticscholar +1 more source
High-throughput proteogenomics of
, 2012 Background The structural and functional annotation of genomes is now heavily based on data obtained using automated pipeline systems. The key for an accurate structural annotation consists of blending similarities between closely related genomes with ...
Christie-Oleza Joseph A +10 more
core +1 more source
Frontiers in Immunology, 2022 Increasing the number of CpG dinucleotides in RNA viral genomes, while preserving the original amino acid composition, leads to impaired infection which does not cause disease.
D. Udenze +3 more
semanticscholar +1 more source
Strategies to identify and edit improvements in synthetic genome segments episomally
bioRxiv, 2022 Genome engineering projects often utilize bacterial artificial chromosomes (BACs) to carry multi-kilobase DNA segments on low copy number vectors. However, DNA synthesis and amplification have the potential to impose mutations on the contained DNA ...
Alexandra Rudolph +5 more
semanticscholar +1 more source
Systematic Evolution and Study of UAGN Decoding tRNAs in a Genomically Recoded Bacteria [PDF]
Scientific Reports, 2016 AbstractWe report the first systematic evolution and study of tRNA variants that are able to read a set of UAGN (N = A, G, U, C) codons in a genomically recoded E. coli strain that lacks any endogenous in-frame UAGN sequences and release factor 1. Through randomizing bases in anticodon stem-loop followed by a functional selection, we identified tRNA ...
Wang, Nanxi +4 more
openaire +2 more sources
PROGRAMMED TRANSLATIONAL RECODING SIGNALS AS A THERAPEUTIC TARGET AGAINST ALPHAVIRUSES [PDF]
, 2018 While infection from communicable diseases has posed a longstanding threat to human health throughout history, the modern realities of population expansion, global travel, and climate change have facilitated the rapid emergence and worldwide distribution
Kendra, Joseph Aaron
core +1 more source
Genomic Structural Variations Provide Insights Into Litter Size and Teat Number Traits in Hu Sheep
Animal Research and One Health, EarlyView.Here, we conducted whole genome sequencing on 300 Hu sheep with an average depth of 16.51X. Two candidate genes associated with litter size and teat number traits were identified, namely MAST2 and AFDN. ABSTRACT Litter size and the teat number are important economic indicators in sheep production.
Xin Xiang +3 more
wiley +1 more source