Results 31 to 40 of about 1,107,385 (165)
Frontiers in Immunology, 2020 Experimental increase of CpG dinucleotides in an RNA virus genome impairs infection providing a promising approach for vaccine development. While CpG recoding is an emerging and promising vaccine approach, little is known about infection phenotypes ...
Ivan Trus +6 more
semanticscholar +1 more source
Limits of variation, specific infectivity, and genome packaging of massively recoded poliovirus genomes [PDF]
Proceedings of the National Academy of Sciences, 2017 Significance We constructed viable poliovirus (PV) variants carrying up to 2,104 synonymous mutations in the ORF. We studied proliferation phenotypes, particularly the specific infectivity ( si ) that defines the probability of a single virion to initiate a single cell infection.
Yutong Song +10 more
openaire +2 more sources
Large-scale recoding of an arbovirus genome to rebalance its insect versus mammalian preference [PDF]
Proceedings of the National Academy of Sciences, 2015 Significance Arboviruses (arthropod-borne viruses), a large group of RNA viruses, replicate in insects that transmit them to mammals, their second host. Insects and mammals have evolved different protein encoding strategies (codon pair bias); hence, arboviruses must delicately balance their encodings between two phyla.
Sam H. Shen +8 more
semanticscholar +3 more sources
Synthetic genome recoding: new genetic codes for new features.
, 2020 Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years.
core +1 more source
, 2021 Genome recoding enables incorporating new functions into the DNA of microorganisms. By reassigning codons to noncanonical amino acids, the generation of new-to-nature proteins offers countless opportunities for bioproduction and biocontainment in ...
Richard van Kranenburg (107687) +4 more
core +1 more source
Synthesis of Recoded Bacterial Genomes toward Bespoke Biocatalysis [PDF]
Trends in Biotechnology, 2019 Ongoing efforts in synthetic biology aim at constructing (micro)organisms with (pre)defined properties. A recent breakthrough is the chemical synthesis of a recoded Escherichia coli genome by Fredens et al. (Nature, 2019). Besides the conceptual and technological tour de force, the consequences of this unprecedented effort for whole-cell biocatalysis ...
openaire +3 more sources
Journal of Virology, 2023 Porcine epidemic diarrhea virus (PEDV) is a deadly coronavirus for neonatal piglets and no effective vaccines are available. Transcriptional regulatory sequences (TRSs) are critical in regulating coronavirus discontinuous transcription.
Xiaoyu Niu +8 more
semanticscholar +1 more source
Elegant and Innovative Recoding Strategies for Advancing Vaccine Development [PDF]
VaccinesFrançois Meurens +2 more
exaly +2 more sources
bioRxiv, 2022 Synonymous recoding of RNA virus genomes is a promising approach for generating attenuated viruses to use as vaccines. Problematically, recoding typically hinders virus growth, but this may be rectified using CpG dinucleotide enrichment.
C. Sharp +14 more
semanticscholar +1 more source
Engineering a genomically recoded organism with one stop codon. [PDF]
NatureThe genetic code is conserved across all domains of life, yet exceptions have revealed variations in codon assignments and associated translation factors1-3. Inspired by this natural malleability, synthetic approaches have demonstrated whole-genome replacement of synonymous codons to construct genomically recoded organisms (GROs)4,5 with alternative ...
Grome MW +17 more
europepmc +3 more sources