Results 1 to 10 of about 732,701 (348)

Characterisation of a stably integrated expression system for exogenous protein expression in DT40 [version 1; referees: 2 approved, 1 approved with reservations]

Wellcome Open Research, 2017
The use of constitutive promoters to drive exogenous protein expression is an important tool for the study of diverse biological processes. To create and characterise a stably integrated expression system for DT40 cells, we constructed integration ...
Meliti Skouteri, Helfrid Hochegger, Antony M. Carr   +2 more
doaj   +1 more source

Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx [PDF]

, 2017
The genomes of most bacteria contain mobile DNA elements that can contribute to undesirable genetic instability in engineered cells. In particular, transposable insertion sequence (IS) elements can rapidly inactivate genes that are important for a ...
Barrick, Jeffrey E, Dasgupta, Aurko, Renda, Brian A, Suárez, Gabriel A   +3 more
core   +2 more sources

ATM Localization and Heterochromatin Repair Depend on Direct Interaction of the 53BP1-BRCT2 Domain with γH2AX

Cell Reports, 2015
53BP1 plays multiple roles in mammalian DNA damage repair, mediating pathway choice and facilitating DNA double-strand break repair in heterochromatin. Although it possesses a C-terminal BRCT2 domain, commonly involved in phospho-peptide binding in other
Robert A. Baldock, Matthew Day, Oliver J. Wilkinson, Ross Cloney, Penelope A. Jeggo, Antony W. Oliver, Felicity Z. Watts, Laurence H. Pearl   +7 more
doaj   +1 more source

Molecular Basis for DNA Double-Strand Break Annealing and Primer Extension by an NHEJ DNA Polymerase

Cell Reports, 2013
Nonhomologous end-joining (NHEJ) is one of the major DNA double-strand break (DSB) repair pathways. The mechanisms by which breaks are competently brought together and extended during NHEJ is poorly understood.
Nigel C. Brissett, Maria J. Martin, Edward J. Bartlett, Julie Bianchi, Luis Blanco, Aidan J. Doherty   +5 more
doaj   +1 more source

Monoubiquitination by the human Fanconi anemia core complex clamps FANCI:FANCD2 on DNA in filamentous arrays

eLife, 2020
FANCI:FANCD2 monoubiquitination is a critical event for replication fork stabilization by the Fanconi anemia (FA) DNA repair pathway. It has been proposed that at stalled replication forks, monoubiquitinated-FANCD2 serves to recruit DNA repair proteins ...
Winnie Tan, Sylvie van Twest, Andrew Leis, Rohan Bythell-Douglas, Vincent J Murphy, Michael Sharp, Michael W Parker, Wayne Crismani, Andrew J Deans   +8 more
doaj   +1 more source

Inhibition of topoisomerase 2 catalytic activity impacts the integrity of heterochromatin and repetitive DNA and leads to interlinks between clustered repeats

Nature Communications
DNA replication and transcription generate DNA supercoiling, which can cause topological stress and intertwining of daughter chromatin fibers, posing challenges to the completion of DNA replication and chromosome segregation.
Michalis Amoiridis, John Verigos, Karen Meaburn, William H. Gittens, Tao Ye, Matthew J. Neale, Evi Soutoglou   +6 more
doaj   +1 more source

ATR-Mediated FANCI Phosphorylation Regulates Both Ubiquitination and Deubiquitination of FANCD2

Frontiers in Cell and Developmental Biology, 2020
DNA interstrand crosslinks (ICLs) are a physical barrier to replication and therefore toxic to cell viability. An important mechanism for the removal of ICLs is the Fanconi Anemia DNA repair pathway, which is initiated by mono-ubiquitination of FANCD2 ...
Winnie Tan, Winnie Tan, Sylvie van Twest, Vincent J. Murphy, Andrew J. Deans, Andrew J. Deans   +5 more
doaj   +1 more source

A 212-nt long RNA structure in the Tobacco necrosis virus-D RNA genome is resistant to Xrn degradation [PDF]

, 2019
Plus-strand RNA viruses can accumulate viral RNA degradation products during infections. Some of these decay intermediates are generated by the cytosolic 5′-to-3′ exoribonuclease Xrn1 (mammals and yeast) or Xrn4 (plants) and are formed when the enzyme ...
Chaminda, Gunawardene D., Newburn, Laura R., White, K. Andrew   +2 more
core   +1 more source

A novel role for the peptidyl-prolyl cis-trans isomerase Cyclophilin A in DNA-repair following replication fork stalling via the MRE11-RAD50-NBS1 complex

EMBO Reports
Cyclosporin A (CsA) induces DNA double-strand breaks in LIG4 syndrome fibroblasts, specifically upon transit through S-phase. The basis underlying this has not been described.
Marisa Bedir, Emily Outwin, Rita Colnaghi, Lydia Bassett, Iga Abramowicz, Mark O’Driscoll   +5 more
doaj   +1 more source

Characterisation of a stably integrated expression system for exogenous protein expression in DT40 [version 2; referees: 2 approved, 1 approved with reservations]

Wellcome Open Research, 2017
The use of constitutive promoters to drive exogenous protein expression is an important tool for the study of diverse biological processes. To create and characterise a stably integrated expression system for DT40 cells, we constructed integration ...
Meliti Skouteri, Helfrid Hochegger, Antony M. Carr   +2 more
doaj   +1 more source