Integrated RPA-CRISPR/Cas12a Technology for Rapid Detection of <i>Salmonella enterica</i>. [PDF]
Akimbekova A +4 more
europepmc +1 more source
Plant species richness was greater in summer in both alpine and subalpine grassland ecosystems. Soil moisture demonstrates significant positive correlations with soil organic carbon and total nitrogen across seasonal periods. Microbial α‐diversity peaks during summer but maintains functional stability across seasons.
Huma Ali +4 more
wiley +1 more source
Asymmetric LAMP-gold nanoparticle biosensing for rapid detection of Kenyan tomato leaf curl virus isolates from crude extracts. [PDF]
Ndudzo A +3 more
europepmc +1 more source
Application of CRISPR-Cas12a/Cas13a dual enzyme cleavage technology in the differential diagnosis of Chikungunya virus and Dengue virus. [PDF]
Jiang T, Guo Z, Wang Z.
europepmc +1 more source
Computational Intelligence Accelerates CRISPR‐Cas‐Based Diagnostic Design
Computational intelligence, including pangenome‐guided target mining, machine‐learning‐based guide RNA optimization, and autonomous AI agents, is shifting CRISPR diagnostic design from empirical screening to systematic, data‐driven engineering and accelerating the path from pathogen discovery to deployable diagnostics.
Hengyu Wang +3 more
wiley +1 more source
Enhanced Early Detection and Precision Monitoring of Rubber Tree Powdery Mildew Pathogen <i>Erysiphe quercicola</i> Using Quantitative PCR and Droplet Digital PCR. [PDF]
Liang X +7 more
europepmc +1 more source
Prebiotic Ginseng polysaccharides (GP) alleviate mastitis through selective enrichment of gut L. murinus, which elevates its anti‐inflammatory metabolite deoxycholic acid (DCA). Circulating DCA engages mammary epithelial TGR5 receptors, triggering the cAMP–PKA pathway to suppress NF‐κB/NLRP3‐mediated inflammation.
Zhijie Zheng +15 more
wiley +1 more source
Transcriptomic and RNA Modification Landscape of Severe Fever with Thrombocytopenia Syndrome Virus Revealed by Nanopore Direct RNA Sequencing. [PDF]
Yuan H +9 more
europepmc +1 more source
The “efficacy paradox” of phytochemicals, low bioavailability yet significant health benefits, is associated with gut microbiota, which biotransforms dietary precursors into bioactive metabolites, enabling systemic effects. Thus, health outcomes of diet depend not just on intake “the rainbow”, but on gut microbial metabolism, redefining “you are what ...
Peng Yang, Ren‐You Gan
wiley +1 more source
One-Pot LAMP-Coupled CRISPR/Cas12b Assay Enables Sensitive Detection of <i>Helicobacter pylori</i>. [PDF]
Tang Z +6 more
europepmc +1 more source

