Results 21 to 30 of about 16,273 (193)

Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.). [PDF]

PLoS ONE, 2015
Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts.
Roberta Fogliatto Mariot, Luisa Abruzzi de Oliveira, Marleen M Voorhuijzen, Martijn Staats, Ronald C B Hutten, Jeroen P Van Dijk, Esther Kok, Jeverson Frazzon   +7 more
doaj   +1 more source

Transcriptome-Based Selection and Validation of Reference Genes for Gene Expression Analysis of Alicyclobacillus acidoterrestris Under Acid Stress

Frontiers in Microbiology, 2021
Alicyclobacillus acidoterrestris is a major concern in fruit juice industry due to its spoilage potential of acidic fruit juice. Quantifying the expression levels of functional genes by real-time quantitative polymerase chain reaction (RT-qPCR) is ...
Ning Zhao, Junnan Xu, Lingxia Jiao, Mengzhen Qiu, Jie Zhang, Xinyuan Wei, Mingtao Fan   +6 more
doaj   +1 more source

Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato ‘Hawaii 7996’ interactions

Scientific Reports, 2021
Reverse transcription-quantitative PCR (RT-qPCR) is an analytical tool for gene expression quantification. Reference genes are not yet available for gene expression analysis during interactions of Ralstonia solanacearum with ‘Hawaii 7996’ (the most ...
Greecy M. R. Albuquerque, Fernando C. A. Fonseca, Leonardo S. Boiteux, Rafaela C. F. Borges, Robert N. G. Miller, Carlos A. Lopes, Elineide B. Souza, Maria Esther N. Fonseca   +7 more
doaj   +1 more source

Genetic determinants of mycotoxin synthesis in genus Fusarium

Czech Journal of Food Sciences, 2011
The fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites - mycotoxins.
Hana Havránková, Jarmila Pazlarová, Jaroslava Ovesná   +2 more
doaj   +1 more source

Identification of stable endogenous reference genes for real-time PCR in the human fetal gonad using an external standard technique [PDF]

, 2011
Peer ...
Ana Monteiro, Andersen, Baker, Bower, Ellefsen, Huggett, Johnson, O'Shaughnessy, O'Shaughnessy, Paul A. Fowler, Peter J. O'Shaughnessy, Rajapaksha, Smith, Vandesompele   +13 more
core   +1 more source

Evaluation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in Pyrus pyrifolia using different tissue samples and seasonal conditions.

PLoS ONE, 2014
We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α ...
Tsuyoshi Imai, Benjamin E Ubi, Takanori Saito, Takaya Moriguchi   +3 more
doaj   +1 more source

Modulation of the host Th1 immune response in pigeon protozoal encephalitis caused by Sarcocystis calchasi [PDF]

, 2013
Pigeon protozoal encephalitis (PPE) is an emerging central-nervous disease of domestic pigeons (Columba livia f. domestica) reported in Germany and the United States.
Gruber, Achim D., Kaspers, Bernd, Klopfleisch, Robert, Lierz, Michael, Meyer, Anne, Olias, Philipp   +5 more
core   +3 more sources

Validation of Reference Genes for Gene Expression Studies by RT-qPCR in HepaRG Cells during Toxicity Testing and Disease Modelling

Cells, 2020
Gene expression analysis by quantitative real-time polymerase chain reaction (RT-qPCR) is routinely used in biomedical studies. The reproducibility and reliability of the data fundamentally depends on experimental design and data interpretation.
Joanna Brzeszczyńska, Filip Brzeszczyński, Kay Samuel, Katie Morgan, Steven D. Morley, John N. Plevris, Peter C. Hayes   +6 more
doaj   +1 more source

MicroRNA profiling in plasma samples using qPCR arrays: Recommendations for correct analysis and interpretation. [PDF]

PLoS ONE, 2018
MicroRNA (miRNA) regulate gene expression through posttranscriptional mRNA degradation or suppression of translation. Many (pre)analytical issues remain to be resolved for miRNA screening with TaqMan Low Density Arrays (TLDA) in plasma samples, such as ...
Andreas B Gevaert, Isabel Witvrouwen, Christiaan J Vrints, Hein Heidbuchel, Emeline M Van Craenenbroeck, Steven J Van Laere, Amaryllis H Van Craenenbroeck   +6 more
doaj   +1 more source

Prawn Shell Chitosan Has Anti-Obesogenic Properties, Influencing Both Nutrient Digestibility and Microbial Populations in a Pig Model [PDF]

, 2015
This study was supported financially (Grant-Aid Agreement No. MFFRI/07/01) under the Sea Change Strategy with the support of the Marine Institute and the Department of Agriculture, Food and the Marine, funded under the National Development Plan 2007 ...
Egan, Aine M., Hayes, Maria, O'Doherty, John V., Sweeney, Torres   +3 more
core   +1 more source