Results 21 to 30 of about 16,155 (172)

Validation of suitable internal control genes for expression studies in aging. [PDF]

, 2009
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA, Burkle, A, Caiafa, Paola, Calabrese, Roberta, Chevanne, M, Ciccarone, Fabio, Guastafierro, Tiziana, MORENO VILLANUEVA, M, Reale, Anna, Zampieri, Michele   +9 more
core   +1 more source

Reference gene selection and RNA preservation protocol in the cat flea, Ctenocephalides felis, for gene expression studies [PDF]

, 2016
This work was supported by a Knowledge Transfer Network BBSRC Industrial Case (#414 BB/L502467/1) studentship in association Zoetis Inc.Peer ...
Baird, John, Bowman, Alan S, Campbell, Ewan M, McIntosh, Catriona H, Woods, Debra J., Zinser, Erich   +5 more
core   +1 more source

Methylation-dependent PAD2 upregulation in multiple sclerosis peripheral blood [PDF]

, 2012
Background: Peptidylarginine deiminase 2 (PAD2) and peptidylarginine deiminase 4 (PAD4) are two members of PAD family which are over-expressed in the multiple sclerosis (MS) brain.
ANNIBALI, Viviana, CAIAFA, Paola, CALABRESE, ROBERTA, CICCARONE, FABIO, COARELLI, GIULIA, GUASTAFIERRO, Tiziana, MECHELLI, Rosella, SALVETTI, Marco, UMETON, RENATO, ZAMPIERI, Michele   +9 more
core   +1 more source

Genetic determinants of mycotoxin synthesis in genus Fusarium

Czech Journal of Food Sciences, 2011
The fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites - mycotoxins.
Hana Havránková, Jarmila Pazlarová, Jaroslava Ovesná   +2 more
doaj   +1 more source

Identification of stable endogenous reference genes for real-time PCR in the human fetal gonad using an external standard technique [PDF]

, 2011
Peer ...
Ana Monteiro, Andersen, Baker, Bower, Ellefsen, Huggett, Johnson, O'Shaughnessy, O'Shaughnessy, Paul A. Fowler, Peter J. O'Shaughnessy, Rajapaksha, Smith, Vandesompele   +13 more
core   +1 more source

Evaluation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in Pyrus pyrifolia using different tissue samples and seasonal conditions.

PLoS ONE, 2014
We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α ...
Tsuyoshi Imai, Benjamin E Ubi, Takanori Saito, Takaya Moriguchi   +3 more
doaj   +1 more source

Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus [PDF]

, 2008
Background During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research.
A Bas, A Radonic, A Szabo, A Zapata, AL Filby, B Liss, C Ramakers, CL Andersen, EO Koppang, F Haller, G Rauch, HC Ingerslev, J Vandesompele, Joern P Scharsack, JP Scharsack, K Pechhold, KM Wegner, MF Oleksiak, MW Pfaffl, N Nicot, PA Olsvik, S Becker, SA Bustin, SA Bustin, Sascha Hibbeler, SF Gonzalez, SM Jorgensen, Sven Becker, TBH Reusch, V Ransbotyn   +29 more
core   +3 more sources

Validation of Reference Genes for Gene Expression Studies by RT-qPCR in HepaRG Cells during Toxicity Testing and Disease Modelling

Cells, 2020
Gene expression analysis by quantitative real-time polymerase chain reaction (RT-qPCR) is routinely used in biomedical studies. The reproducibility and reliability of the data fundamentally depends on experimental design and data interpretation.
Joanna Brzeszczyńska, Filip Brzeszczyński, Kay Samuel, Katie Morgan, Steven D. Morley, John N. Plevris, Peter C. Hayes   +6 more
doaj   +1 more source

MicroRNA profiling in plasma samples using qPCR arrays: Recommendations for correct analysis and interpretation. [PDF]

PLoS ONE, 2018
MicroRNA (miRNA) regulate gene expression through posttranscriptional mRNA degradation or suppression of translation. Many (pre)analytical issues remain to be resolved for miRNA screening with TaqMan Low Density Arrays (TLDA) in plasma samples, such as ...
Andreas B Gevaert, Isabel Witvrouwen, Christiaan J Vrints, Hein Heidbuchel, Emeline M Van Craenenbroeck, Steven J Van Laere, Amaryllis H Van Craenenbroeck   +6 more
doaj   +1 more source

Modulation of the host Th1 immune response in pigeon protozoal encephalitis caused by Sarcocystis calchasi [PDF]

, 2013
Pigeon protozoal encephalitis (PPE) is an emerging central-nervous disease of domestic pigeons (Columba livia f. domestica) reported in Germany and the United States.
Gruber, Achim D., Kaspers, Bernd, Klopfleisch, Robert, Lierz, Michael, Meyer, Anne, Olias, Philipp   +5 more
core   +3 more sources