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Genotyping‐in‐Thousands by sequencing (GT‐seq) panel development and application to minimally invasive DNA samples to support studies in molecular ecology

Molecular Ecology Resources, 2020
Minimally invasive sampling (MIS) is widespread in wildlife studies; however, its utility for massively parallel DNA sequencing (MPS) is limited.
D. A. Schmidt   +4 more
semanticscholar   +1 more source

Multiplexed genotyping with sequence-tagged molecular inversion probes

Nature Biotechnology, 2003
We report on the development of molecular inversion probe (MIP) genotyping, an efficient technology for large-scale single nucleotide polymorphism (SNP) analysis. This technique uses MIPs to produce inverted sequences, which undergo a unimolecular rearrangement and are then amplified by PCR using common primers and analyzed using universal sequence tag
Paul, Hardenbol   +10 more
openaire   +2 more sources

Genotyping-by-Sequencing and Its Application to Oat Genomic Research

2017
Genotyping-by-sequencing (GBS) has emerged as a useful genomic approach for sampling genome-wide genetic variation, performing genome-wide association mapping, and conducting genomic selection. It is a combined one-step process of SNP marker discovery and genotyping through genome reduction with restriction enzymes and SNP calling with or without a ...
Yong-Bi, Fu, Mo-Hua, Yang
openaire   +2 more sources

Genotyping and Sequencing

2020
Teresa Ezponda   +3 more
openaire   +1 more source

The Restriction Enzyme Target Approach to Genotyping by Sequencing (GBS)

2014
The modified genotyping by sequencing method described here emphasizes verifying the success of each library ligation by performing individual PCRs, before preparing the pool of barcoded amplicons to be sequenced. Although this extra step might seem excessive, it will give peace of mind to the researcher knowing each individual is represented in the ...
openaire   +2 more sources

Analysing Genotyping-By-Sequencing (GBS) data

Part of a training course for alpaca breeders, Cusco, Peru (online) Dec 2023Summary:GBS is an attractive tool for genetic studiesAnalyses should account for the data generation process (e.g. allele sampling instead of genotype calls)Our team has developed such methods for:Relatedness, Parentage, Identity, Gender assignmentLinkage, Linkage ...
openaire   +1 more source

Genotyping-by-sequencing: a promising tool for plant genetics research and breeding

Horticulture Environment and Biotechnology, 2017
Yong Suk Chung   +3 more
semanticscholar   +1 more source

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