Results 61 to 70 of about 1,176,096 (345)

Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian Population

Children, 2022
Permanent systemic inflammation is a defining feature of systemic lupus erythematosus (SLE), which affects multiple organs. Gelatinase B/matrix metalloproteinase-9 (MMP-9) is an essential protease investigated in inflammation that has been linked to SLE.
Zeinab R. Attia, Mohamed M. Zedan, Thuraya M. Mutawi, Entsar A. Saad, Rania A. Abd El Azeem, Mohamed A. El Basuni   +5 more
doaj   +1 more source

High throughput SNP discovery and genotyping in hexaploid wheat

PLoS ONE, 2018
Because of their abundance and their amenability to high-throughput genotyping techniques, Single Nucleotide Polymorphisms (SNPs) are powerful tools for efficient genetics and genomics studies, including characterization of genetic resources, genome-wide
Hélène Rimbert, Benoît Darrier, Julien Navarro, Jonathan Kitt, F. Choulet, M. Leveugle, Jorge Duarte, N. Rivière, K. Eversole, J. Le Gouis, Alessandro Davassi, F. Balfourier, M. Le Paslier, A. Bérard, D. Brunel, C. Feuillet, C. Poncet, P. Sourdille, E. Paux   +18 more
semanticscholar   +1 more source

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Evaluation of Different Molecular Markers for Genotyping Non-Saccharomyces Wine Yeast Species

Microbiology Research, 2022
Wine quality is determined by the particular yeast strains prevailing at various stages of fermentation. Therefore, the ability to make an easy, fast, and unambiguous discrimination of yeasts at the strain level is of great importance.
Aspasia Nisiotou, Emanouela Gyftogianni, Georgios Banilas   +2 more
doaj   +1 more source

The GATK joint genotyping workflow is appropriate for calling variants in RNA-seq experiments

Journal of Animal Science and Biotechnology, 2019
The Genome Analysis Toolkit (GATK) is a popular set of programs for discovering and genotyping variants from next-generation sequencing data. The current GATK recommendation for RNA sequencing (RNA-seq) is to perform variant calling from individual ...
Jean-Simon Brouard, F. Schenkel, A. Marete, N. Bissonnette   +3 more
semanticscholar   +1 more source

Genotyping with TaqMAMA

Genomics, 2004
TaqMAMA combines the quantitative strengths of TaqMan with the allele-specific PCR of MAMA. In this article we develop TaqMAMA as a technique for screening human DNA samples for known genetic polymorphisms. In the first set of experiments, plasmids that model all types of genetic polymorphisms were used to understand the relationship between TaqMAMA ...
David E. Watson, Dong-Jing Fu, Baohui Li, Ibrahim Kadura   +3 more
openaire   +3 more sources

Linked dimers of the AAA+ ATPase Msp1 reveal energetic demands and mechanistic plasticity for substrate extraction from lipid bilayers

FEBS Letters, EarlyView.
Cells must clear mislocalized or faulty proteins from membranes to survive. The AAA+ ATPase Msp1 performs this task, but dissecting how its six subunits work together is challenging. We engineered linked dimers with varied numbers of functional subunits to reveal how Msp1 subunits cooperate and use energy to extract proteins from the lipid bilayer ...
Deepika Gaur, Brian Acquaviva, Baylee A. Smith, Nathan Walker, Isabella Walter, Matthew L. Wohlever   +5 more
wiley   +1 more source

Role of Bioaerosols on the Short-Distance Transmission of Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus (MRSA) in a Chicken Farm Environment

Antibiotics, 2022
Methicillin-resistant Staphylococcus aureus (MRSA) is a dynamic and tenacious pathogenic bacterium which is prevalent in livestock farming environments.
Bing-Mu Hsu, Jung-Sheng Chen, Gwo-Jong Hsu, Suprokash Koner, Viji Nagarajan, Hsin-Chi Tsai   +5 more
doaj   +1 more source

Genotyping Polyploids from Messy Sequencing Data

Genetics, 2018
Gerard et al. highlight several issues encountered when genotyping polyploid organisms from next-generation sequencing data, including allelic bias, overdispersion, and outlying observations.
David Gerard, L. F. V. Ferrão, A. Garcia, M. Stephens   +3 more
semanticscholar   +1 more source

Comparing self‐reported race and genetic ancestry for identifying potential differentially methylated sites in endometrial cancer: insights from African ancestry proportions using machine learning models

Molecular Oncology, EarlyView.
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley   +1 more source