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Current Protocols in Plant Biology, 2017
AbstractGenotyping‐by‐sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high‐throughput sequencing. GBS is a refinement of restriction site–associated DNA sequencing (RADseq) methods, with a goal of being able to perform library preparations quickly, cost ...
Jason G Wallace, Sharon E Mitchell
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AbstractGenotyping‐by‐sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high‐throughput sequencing. GBS is a refinement of restriction site–associated DNA sequencing (RADseq) methods, with a goal of being able to perform library preparations quickly, cost ...
Jason G Wallace, Sharon E Mitchell
exaly +3 more sources
Adversarial Attacks on Genotype Sequences
ICASSP 2023 - 2023 IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP), 2022ABSTRACT Adversarial attacks can drastically change the output of a method by performing a small change on its input. While they can be a useful framework to analyze worst-case robustness, they can also be used by malicious agents to perform damage in machine learning-based applications.
Daniel Mas Montserrat +1 more
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Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny [PDF]
, 2020 Genotyping-by-sequencing (GBS) is a method to discover and genotype simultaneous genome-wide high-throughput single nucleotide polymorphisms (SNPs). GBS is based on reducing genome complexity with restriction enzymes. Here we describe a method developed by Elshire et al.
Favre, Félicien +3 more
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Methods for Genotyping-by-Sequencing
2016A major goal for biologists is to understand the connection between genes and phenotypic traits, and genetic mapping in experimental populations remains a powerful approach for discovering the causal genes underlying phenotypes. For genetic mapping, the process of genotyping was previously a major rate-limiting step.
Rowan, B. +4 more
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Skim-Based Genotyping by Sequencing
2014Genotyping by sequencing (GBS) is a relatively new method used to determine the differences in the genetic makeup of individuals. Its novelty stems from a combination of two already available methods: genotyping and next-generation sequencing. Depending on the individual study design GBS protocols can take multiple forms, however most share a sequence ...
Golicz, Agnieszka A. +2 more
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Genotyping and Genomic Sequencing in Clinical Practice
Clinics in Liver Disease, 2007The global prevalence of chronic hepatitis B and its associated serious sequelae demand technologically advanced techniques of management. Nucleic acid testing (NAT) plays a key role in the diagnosis, surveillance, and treatment of chronic hepatitis B.
Robert G, Gish, Stephen, Locarnini
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Genotyping-by-Sequencing in Potato
2017Genotyping-by-sequencing (GBS) is one of the most cost-effective approaches to sequencing in potato (SNP) discovery and genotyping. The reduction of genome complexity that is central to the GBS approach is useful in the analysis of many plant genomes in which large size and polyploidy can prove challenging.
Chiheb Boudhrioua +6 more
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Hepatitis B virus genotype A: design of reference sequences for sub-genotypes
Virus Genes, 2016Genotype A of hepatitis B virus (HBV/A) is widespread and is currently divided into six sub-genotypes. Suitable reference sequences for different sub-genotypes can facilitate research on HBV/A. However, the current reference sequences for this virus are insufficient.
Qun, Cai +4 more
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Genotyping-by-Sequencing on the Ion Torrent Platform in Barley
2018The characterization of genetic polymorphism is a crucial step in both genetic studies and breeding programs. Genotyping-by-sequencing (GBS) constitutes one of the most attractive approaches for this purpose, especially in a genome as large as that of barley.
Abed, Amina +5 more
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Targeted Amplicon Genotyping by Sanger Sequencing
This chapter presents a protocol for Sanger sequencing, a conventional yet rapid, cost-effective, and highly accurate method for targeted sequencing. It involves designing specific primers for forward and reverse template extension reactions, during which fluorophore labeled, chain terminating dideoxynucleotides are incorporated into newly replicated ...Anthony Torres, Reginald Gaudino
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