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Genotyping-by-Sequencing in Potato
2017Genotyping-by-sequencing (GBS) is one of the most cost-effective approaches to sequencing in potato (SNP) discovery and genotyping. The reduction of genome complexity that is central to the GBS approach is useful in the analysis of many plant genomes in which large size and polyploidy can prove challenging.
Chiheb Boudhrioua +6 more
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Genotyping-by-Sequencing on the Ion Torrent Platform in Barley
2018The characterization of genetic polymorphism is a crucial step in both genetic studies and breeding programs. Genotyping-by-sequencing (GBS) constitutes one of the most attractive approaches for this purpose, especially in a genome as large as that of barley.
Abed, Amina +5 more
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Hepatitis B virus genotype A: design of reference sequences for sub-genotypes
Virus Genes, 2016Genotype A of hepatitis B virus (HBV/A) is widespread and is currently divided into six sub-genotypes. Suitable reference sequences for different sub-genotypes can facilitate research on HBV/A. However, the current reference sequences for this virus are insufficient.
Qun, Cai +4 more
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DNA sequencing and genotyping in miniaturized electrophoresis systems
ELECTROPHORESIS, 2004AbstractAdvances in microchannel electrophoretic separation systems for DNA analyses have had important impacts on biological and biomedical sciences, as exemplified by the successes of the Human Genome Project (HGP). As we enter a new era in genomic science, further technological innovations promise to provide other far‐reaching benefits, many of ...
Cheuk-Wai, Kan +3 more
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The Use of the MegaBACE for Sequencing and Genotype Analysis
2013Despite the advent of next generation sequencing techniques, which provide access to an enormous amount of genomic information in a relatively short time, the conventional Sanger sequencing and microsatellite genotyping analyses present a straightforward method to answer clearly defined questions in population genetics, phylogeography, or forensics ...
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Targeted Amplicon Genotyping by Sanger Sequencing
This chapter presents a protocol for Sanger sequencing, a conventional yet rapid, cost-effective, and highly accurate method for targeted sequencing. It involves designing specific primers for forward and reverse template extension reactions, during which fluorophore labeled, chain terminating dideoxynucleotides are incorporated into newly replicated ...Anthony Torres, Reginald Gaudino
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Genotyping of Nocardia farcinica with multilocus sequence typing
European Journal of Clinical Microbiology & Infectious Diseases, 2016Nocardia are aerobic Gram-positive saprophytes that are widely distributed in nature, but some species cause nocardiosis, especially opportunistic infections that affect immunocompromised patients mostly. In this study, we developed a multilocus sequence typing (MLST) scheme using seven housekeeping genes (gyrB, hsp65, secA1, rpoB, rpoA, recA, and trpB)
P, Du +8 more
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Integrating targeted genetic markers to genotyping-by-sequencing for an ultimate genotyping tool
Theoretical and Applied GeneticsNew selection methods, using trait-specific markers (marker-assisted selection (MAS)) and/or genome-wide markers (genomic selection (GS)), are becoming increasingly widespread in breeding programs. This new era requires innovative and cost-efficient solutions for genotyping.
Maxime de Ronne +13 more
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