Optimization of Giant Unilamellar Vesicle Electroformation for Phosphatidylcholine/Sphingomyelin/Cholesterol Ternary Mixtures [PDF]
Membranes, 2022 Artificial vesicles are important tools in membrane research because they enable studying membrane properties in controlled conditions. Giant unilamellar vesicles (GUVs) are specially interesting due to their similarity in size to eukaryotic cells.
Zvonimir Boban +4 more
doaj +3 more sources
High-Speed Imaging of Giant Unilamellar Vesicle Formation in cDICE [PDF]
ACS OmegaGiant unilamellar vesicles (GUVs) are widely used as in vitro model membranes in biophysics and as cell-sized containers in synthetic biology. Despite their ubiquitous use, there is no one-size-fits-all method for their production.
Lori Van de Cauter +7 more
doaj +3 more sources
Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results [PDF]
Membranes, 2021 Since its inception more than thirty years ago, electroformation has become the most commonly used method for growing giant unilamellar vesicles (GUVs). Although the method seems quite straightforward at first, researchers must consider the interplay of ...
Zvonimir Boban +3 more
doaj +3 more sources
Introducing micrometer-sized artificial objects into live cells: a method for cell-giant unilamellar vesicle electrofusion. [PDF]
PLoS ONE, 2014 Here, we report a method for introducing large objects of up to a micrometer in diameter into cultured mammalian cells by electrofusion of giant unilamellar vesicles.
Akira C Saito +4 more
doaj +3 more sources
Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis. [PDF]
Int J Mol Sci, 2023 Artificial cells are based on dynamic compartmentalized systems. Thus, remodeling of membrane-bound systems, such as giant unilamellar vesicles, is finding applications beyond biological studies, to engineer cell-mimicking structures.
Caliari A +4 more
europepmc +2 more sources
Temperature-Promoted Giant Unilamellar Vesicle (GUV) Aggregation: A Way of Multicellular Formation [PDF]
Current Issues in Molecular Biology, 2023 The evolution of unicellular to multicellular life is considered to be an important step in the origin of life, and it is crucial to study the influence of environmental factors on this process through cell models in the laboratory.
Xinmao Wang +4 more
doaj +2 more sources
One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells. [PDF]
ACS Synth Biol, 2019 Here, we introduce a one-pot method for the bottom-up assembly of complex single- and multicompartment synthetic cells. Cellular components are enclosed within giant unilamellar vesicles (GUVs), produced at the milliliter scale directly from small ...
Göpfrich K +6 more
europepmc +2 more sources
Shape Transformations of Lipid Vesicles by Insertion of Bulky-Head Lipids. [PDF]
PLoS ONE, 2015 Lipid vesicles, in particular Giant Unilamellar Vesicles (GUVs), have been increasingly important as compartments of artificial cells to reconstruct living cell-like systems in a bottom-up fashion.
Soichiro Tsuda +4 more
doaj +6 more sources
Large and Giant Unilamellar Vesicle(s) Obtained by Self-Assembly of Poly(dimethylsiloxane)-b-poly(ethylene oxide) Diblock Copolymers, Membrane Properties and Preliminary Investigation of their Ability to Form Hybrid Polymer/Lipid Vesicles. [PDF]
Polymers (Basel), 2019 In the emerging field of hybrid polymer/lipid vesicles, relatively few copolymers have been evaluated regarding their ability to form these structures and the resulting membrane properties have been scarcely studied.
Fauquignon M +5 more
europepmc +2 more sources
Bio-Protocol, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen +1 more
doaj +2 more sources