Results 31 to 40 of about 5,077 (168)

Reconstitution of proteins on electroformed giant unilamellar vesicles [PDF]

, 2015
In vitro reconstitution of simplified biological systems from molecular parts has proven to be a powerful method for investigating the biochemical and biophysical principles underlying cellular processes. In recent years, there has been a growing interest in reconstitution of protein-membrane interactions to understand the critical role played by ...
Schmid, E., Richmond, D., Fletcher, D.
openaire   +3 more sources

Optimization of cryo-electron microscopy for quantitative analysis of lipid bilayers

Biophysical Reports, 2023
Cryogenic electron microscopy (cryo-EM) is among the most powerful tools available for interrogating nanoscale structure of biological materials.
Frederick A. Heberle, Doug Welsch, Haden L. Scott, M. Neal Waxham   +3 more
doaj   +1 more source

Membrane Remodeling by Arc/Arg3.1

Frontiers in Molecular Biosciences, 2021
The activity-regulated cytoskeletal-associated protein (Arc, also known as Arg3.1) is an immediate early gene product induced by activity/experience and required for multiple modes of synaptic plasticity.
Per Niklas Hedde, Per Niklas Hedde, Leonel Malacrida, Leonel Malacrida, Barbara Barylko, Derk D. Binns, Joseph P. Albanesi, David M. Jameson   +7 more
doaj   +1 more source

Rapid preparation of giant unilamellar vesicles. [PDF]

Proceedings of the National Academy of Sciences, 1996
We report here a rapid evaporation method that produces in high yield giant unilamellar vesicles up to 50 microns in diameter. The vesicles are obtained after only 2 min and can be prepared from different phospholipids, including L-alpha-phosphatidylcholine (lecithin), dipalmitoleoyl L-alpha-phosphatidylcholine, and beta-arachidonoyl gamma-palmitoyl L ...
A, Moscho, O, Orwar, D T, Chiu, B P, Modi, R N, Zare   +4 more
openaire   +2 more sources

Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol

Molecules, 2015
In the present investigation we studied the molecular mechanisms of the monodesmosidic saponin digitonin on natural and artificial membranes. We measured the hemolytic activity of digitonin on red blood cells (RBCs). Also different lipid membrane models (
Ikhwan Resmala Sudji, Yamunadevi Subburaj, Nataliya Frenkel, Ana J. García-Sáez, Michael Wink   +4 more
doaj   +1 more source

Study on the Application of Electric Field to Giant Vesicles Comprised of 1,2-Dilauroyl-Sn-Glycero-3-Phosphatidylcholine Using Optical Fluorescence Microscopy [PDF]

Materials Research, 2017
The influence of alternating electric field (AC) in the structure and dynamics of giant unilamellar vesicles (GUVs) comprised of 1,2-dilauroyl-sn-glycero-3-phosphatidylcholine (DLPC) is reported.
Yasmine Miguel Serafini Micheletto, Nádya Pesce da Silveira, Alexandre Gonçalves Dal-Bó, Fernando Carlos Giacomelli, Newton Luiz Dias Filho, Tiago Elias Allievi Frizon, Carlos Manuel Marques, André Pierre Schroder   +7 more
doaj   +1 more source

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles [PDF]

Journal of Visualized Experiments, 2017
The reshaping of the cell membrane is an integral part of many cellular phenomena, such as endocytosis, trafficking, the formation of filopodia, etc. Many different proteins associate with curved membranes because of their ability to sense or induce membrane curvature.
Prévost, Coline, Tsai, Feng-Ching, Bassereau, Patricia, Simunovic, Mijo   +3 more
openaire   +2 more sources

Effects of membrane potentials on the electroporation of giant unilamellar vesicles

PLOS ONE, 2023
Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive technique that utilizes precisely controlled electric field pulses of micro- to millisecond durations to
Abdul Wadud, Mohammad Abu Sayem Karal, Md. Moniruzzaman, Md. Mamun Or Rashid   +3 more
openaire   +3 more sources

Interaction of apolipoprotein A-I in three different conformations with palmitoyl oleoyl phosphatidylcholine vesicles

Journal of Lipid Research, 2002
Interactions of apolipoprotein A-I (apoA-I) with cell membranes appear to be important in the initial steps of reverse cholesterol transport. The objective of this work was to examine the effect of three distinct conformations of apoA-I (lipid-free and ...
M. Alejandra Tricerri, Susana A. Sanchez, Cristina Arnulphi, Diane M. Durbin, Enrico Gratton, Ana Jonas   +5 more
doaj   +1 more source

Stepwise remodeling and subcompartment formation in individual vesicles by three ESCRT-III proteins

iScience, 2023
Summary: The endosomal sorting complex required for transport (ESCRT) is a multi-protein machinery involved in several membrane remodeling processes. Different approaches have been used to resolve how ESCRT proteins scission membranes.
Yunuen Avalos-Padilla, Vasil N. Georgiev, Eleanor Ewins, Tom Robinson, Esther Orozco, Reinhard Lipowsky, Rumiana Dimova   +6 more
doaj   +1 more source