Results 1 to 10 of about 3,495 (182)

Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

Bio-protocol, 2020
Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris, Unai Silvan
exaly   +3 more sources

Reconstitution of Active Plant H+-ATPase AHA2 in Giant Unilamellar Vesicles [PDF]

Bio-Protocol
Membrane transporters mediate the selective movement of ions and molecules across biological membranes and are essential for cellular homeostasis. However, their functional characterization in living cells is often complicated by the complexity of the ...
Huriye Uzun, Thomas Pomorski
doaj   +2 more sources

Dimensions, stability, and deformability of DOPC-cholesterol giant unilamellar vesicles formed by droplet transfer [version 3; peer review: 2 approved, 2 approved with reservations] [PDF]

Open Research Europe
Background Understanding cell membrane-like lipid bilayers is crucial for studying fundamental biological mechanisms. Giant Unilamellar Vesicles (GUVs) are key tools for this investigation and have applications in both synthetic biology and, more ...
Stefano Palagi, Elisa Roberti, Elisa Linda Petrocelli, Dario Cecchi   +3 more
doaj   +2 more sources

Mechanisms of Self-Assembly of Giant Unilamellar Vesicles in the Army Liposome Formulation (ALF) Family of Vaccine Adjuvants [PDF]

Pharmaceutics
Background/Objectives: Army Liposome Formulation with QS21 (ALFQ) is a vaccine adjuvant formulation consisting of liposomes that contain saturated zwitterionic and anionic phospholipids, 55 mol% cholesterol, and small molar amounts of monophosphoryl ...
Calin Nicolescu, Essie Komla, Mangala Rao, Gary R. Matyas, Carl R. Alving   +4 more
doaj   +2 more sources

A new purification technique to obtain specific size distribution of giant lipid vesicles using dual filtration.

PLoS ONE, 2021
A new purification technique is developed for obtaining distribution of giant unilamellar vesicles (GUVs) within a specific range of sizes using dual filtration. The GUVs were prepared using well known natural swelling method.
Mohammad Abu Sayem Karal, Tawfika Nasrin, Marzuk Ahmed, Md Kabir Ahamed, Shareef Ahammed, Salma Akter, Sharif Hasan, Zaid Bin Mahbub   +7 more
doaj   +1 more source

Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles

Advanced Science, 2023
In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Jorik Waeterschoot, Willemien Gosselé, Hojjat Alizadeh Zeinabad, Jeroen Lammertyn, Erin Koos, Xavier Casadevall i Solvas   +5 more
doaj   +1 more source

Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)

Scientific Reports, 2021
Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery ...
Diogo A. Pereira, Alexandre D. Silva, Patricia A. T. Martins, Ana P. Piedade, Dmitro Martynowych, David Veysset, Maria João Moreno, Carlos Serpa, Keith A. Nelson, Luis G. Arnaut   +9 more
doaj   +1 more source

Whole-GUV patch-clamping [PDF]

Proceedings of the National Academy of Sciences, 2016
Significance Although membrane composition and tension modulate the activity of ion channels and transporters, this protein–membrane coupling has been challenging to study due to the difficulty of controlling membrane properties in cells and technical limitations of existing in vitro systems. This work demonstrates that the whole-cell
Garten, Matthias, Mosgaard, Lars D., Bornschlögl, Thomas, Dieudonné, Stéphane, Bassereau, Patricia, Toombes, Gilman E. S.   +5 more
openaire   +3 more sources

Methods to mechanically perturb and characterize GUV-based minimal cell models

Computational and Structural Biotechnology Journal, 2023
Cells shield organelles and the cytosol via an active boundary predominantly made of phospholipids and membrane proteins, yet allowing communication between the intracellular and extracellular environment.
Nadab H. Wubshet, Allen P. Liu
doaj   +1 more source

Influence of sugar concentration on the vesicle compactness, deformation and membrane poration induced by anionic nanoparticles.

PLoS ONE, 2022
Sugar plays a vital role in the structural and functional characteristics of cells. Hence, the interaction of NPs with cell membranes in the presence of sugar concentrations is important for medicinal and pharmacological innovations.
Sharif Hasan, Mohammad Abu Sayem Karal, Salma Akter, Marzuk Ahmed, Md Kabir Ahamed, Shareef Ahammed   +5 more
doaj   +2 more sources