Results 21 to 30 of about 3,495 (182)

Temperature controlled transformations of giant unilamellar vesicles of amphiphilic triblock copolymers synthesized via microfluidic mixing

Applied Surface Science Advances, 2021
We report on a simple approach for synthesis of temperature-responsive giant unilamellar vesicles (GUVs) from poly(N-vinylcaprolactam)15-block-poly(dimethylsiloxane)65-block-poly(N-vinylcaprolactam)15 (PVCL15-PDMS65-PVCL15) triblock copolymer and non ...
Yiming Yang, Veronika Kozlovskaya, Maksim Dolmat, Yin Song, Shuo Qian, Volker S. Urban, Donald Cropek, Eugenia Kharlampieva   +7 more
doaj   +1 more source

GeoV: An Open‐Source Software Package for Quantitative Image Analysis of 3D Vesicle Morphologies

Advanced Intelligent Systems, 2023
Bottom‐up synthetic biology has reconstituted processes like adhesion, cortex formation, or division of giant lipid vesicles (GUVs), which all rely on changes in the vesicle morphology.
Yannik Dreher, Jakob Niessner, Andreas Fink, Kerstin Göpfrich   +3 more
doaj   +1 more source

Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles

Pharmaceutics, 2022
In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-
Paola Albanese, Simone Cataldini, Chloe Z.-J. Ren, Nadia Valletti, Jlenia Brunetti, Jack L.-Y. Chen, Federico Rossi   +6 more
doaj   +1 more source

GUV preparation v1

, 2022
GUV preparation for membrane tube assay application
Liv Jensen, Chunmei Chang
openaire   +1 more source

A protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles

STAR Protocols, 2022
Summary: Here, we present a general protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles (GUVs). In this protocol, we use GUVs to mimic Ago1 protein’s phase separation behavior on the membrane through ...
Yuanxiang Zhu, Yajie Gao, Qinmiao Sun, Dahua Chen   +3 more
doaj   +1 more source

Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results

Membranes, 2021
Since its inception more than thirty years ago, electroformation has become the most commonly used method for growing giant unilamellar vesicles (GUVs). Although the method seems quite straightforward at first, researchers must consider the interplay of ...
Zvonimir Boban, Ivan Mardešić, Witold Karol Subczynski, Marija Raguz   +3 more
doaj   +1 more source

Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion

Membranes, 2023
Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special interest to researchers because of their similarity in size to eukaryotic cells. The most commonly used method for GUVs production is electroformation.
Zvonimir Boban, Ivan Mardešić, Sanja Perinović Jozić, Josipa Šumanovac, Witold Karol Subczynski, Marija Raguz   +5 more
doaj   +1 more source

Dataset of asymmetric giant unilamellar vesicles prepared via hemifusion: Observation of anti-alignment of domains and modulated phases in asymmetric bilayers.

Data in Brief, 2021
The data provided with this paper are confocal fluorescence images of symmetric giant unilamellar vesicles (GUVs) and asymmetric giant unilamellar vesicles (aGUVs).
Thais A. Enoki, Joy Wu, Frederick A. Heberle, Gerald W. Feigenson   +3 more
doaj   +1 more source

Membrane localization of actin filaments stabilizes giant unilamellar vesicles against external deforming forces

European Journal of Cell Biology
Actin organization is crucial for establishing cell polarity, which influences processes such as directed cell motility and division. Despite its critical role in living organisms, achieving similar polarity in synthetic cells remains challenging.
Andreas Fink, Sunnatullo Fazliev, Tobias Abele, Joachim P. Spatz, Kerstin Göpfrich, Elisabetta Ada Cavalcanti-Adam   +5 more
doaj   +1 more source

Effects of membrane potentials on the electroporation of giant unilamellar vesicles.

PLoS ONE, 2023
Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive ...
Md Abdul Wadud, Mohammad Abu Sayem Karal, Md Moniruzzaman, Md Mamun Or Rashid   +3 more
doaj   +1 more source