Results 21 to 30 of about 8,576 (213)
Bio-Protocol, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen +1 more
doaj +1 more source
Applied Surface Science Advances, 2021 We report on a simple approach for synthesis of temperature-responsive giant unilamellar vesicles (GUVs) from poly(N-vinylcaprolactam)15-block-poly(dimethylsiloxane)65-block-poly(N-vinylcaprolactam)15 (PVCL15-PDMS65-PVCL15) triblock copolymer and non ...
Yiming Yang +7 more
doaj +1 more source
GeoV: An Open‐Source Software Package for Quantitative Image Analysis of 3D Vesicle Morphologies
Advanced Intelligent Systems, 2023 Bottom‐up synthetic biology has reconstituted processes like adhesion, cortex formation, or division of giant lipid vesicles (GUVs), which all rely on changes in the vesicle morphology.
Yannik Dreher +3 more
doaj +1 more source
Coupling between pore formation and phase separation in charged lipid membranes [PDF]
, 2015 We investigated the effect of charge on the membrane morphology of giant unilamellar vesicles (GUVs) composed of various mixtures containing charged lipids.
Hamada, Tsutomu +5 more
core +2 more sources
Molecular rheometry: direct determination of viscosity in L-o and L-d lipid phases via fluorescence lifetime imaging [PDF]
, 2013 Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge of their physical state and structure. However, mapping the viscosity and diffusion in the membranes of complex composition is currently a non ...
Agnieszka Olzyńska +37 more
core +2 more sources
Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems
Advanced Science, EarlyView.A comprehensive understanding of the interactions between proteins, lipids and glycocalyx components at the immune synapse is still lacking. Here, an artificial and a semi‐artificial model contact system were established to reconstitute the cell‐cell contact in 3D. The model systems enable the examination of macromolecule enrichment or depletion at the
Ragaller F +8 more
europepmc +2 more sources
Bottom-up assembly of functional intracellular synthetic organelles by droplet-based microfluidics
, 2020 Bottom-up synthetic biology has directed most efforts toward the construction of artificial compartmentalized systems that recreate living cell functions in their mechanical, morphological, or metabolic characteristics.
Platzman, I. +3 more
core +1 more source
Specific Adhesion of Membranes Simultaneously Supports Dual Heterogeneities in Lipids and Proteins [PDF]
, 2015 Membrane adhesion is a vital component of many biological processes. Heterogeneities in lipid and protein composition are often associated with the adhesion site. These heterogeneities are thought to play functional roles in facilitating signalling. Here
Gordon, Vernita D. +3 more
core +1 more source
Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles
Pharmaceutics, 2022 In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-
Paola Albanese +6 more
doaj +1 more source
Shaping Giant Membrane Vesicles in 3D-Printed Protein Hydrogel Cages
, 2020 Giant unilamellar phospholipid vesicles are attractive starting points for constructing minimal living cells from the bottom-up. Their membranes are compatible with many physiologically functional modules and act as selective barriers, while retaining a ...
Eto, H. +5 more
core +1 more source