Temperature-Promoted Giant Unilamellar Vesicle (GUV) Aggregation: A Way of Multicellular Formation [PDF]
Current Issues in Molecular Biology, 2023 The evolution of unicellular to multicellular life is considered to be an important step in the origin of life, and it is crucial to study the influence of environmental factors on this process through cell models in the laboratory.
Zhibiao Wang, Maobin Xie, Hai Qiao
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Optimization of Giant Unilamellar Vesicle Electroformation for Phosphatidylcholine/Sphingomyelin/Cholesterol Ternary Mixtures [PDF]
Membranes, 2022 Artificial vesicles are important tools in membrane research because they enable studying membrane properties in controlled conditions. Giant unilamellar vesicles (GUVs) are specially interesting due to their similarity in size to eukaryotic cells.
Zvonimir Boban +4 more
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Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results [PDF]
Membranes, 2021 Since its inception more than thirty years ago, electroformation has become the most commonly used method for growing giant unilamellar vesicles (GUVs). Although the method seems quite straightforward at first, researchers must consider the interplay of ...
Zvonimir Boban +3 more
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Introducing micrometer-sized artificial objects into live cells: a method for cell-giant unilamellar vesicle electrofusion. [PDF]
PLoS ONE, 2014 Here, we report a method for introducing large objects of up to a micrometer in diameter into cultured mammalian cells by electrofusion of giant unilamellar vesicles.
Akira C Saito +4 more
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Rapid Multi-Well Evaluation of Assorted Materials for Hydrogel-Assisted Giant Unilamellar Vesicle Production: Empowering Bottom-Up Synthetic Biology [PDF]
GelsGiant unilamellar vesicles (GUVs) are versatile cell models in biomedical and environmental research. Of the various GUV production methods, hydrogel-assisted GUV production is most easily implemented in a typical biological laboratory. To date, agarose,
Cherng-Wen Darren Tan +2 more
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Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis [PDF]
International Journal of Molecular Sciences, 2023 Masayuki Imai, Martin Hanczyc
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Pharmaceutics, 2023 Army Liposome Formulation with QS21 (ALFQ), a vaccine adjuvant preparation, comprises liposomes containing saturated phospholipids, with 55 mol% cholesterol relative to the phospholipids, and two adjuvants, monophosphoryl lipid A (MPLA) and QS21 saponin.
Mangala Rao, Carl Alving, Gary Matyas
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Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]
Scientific ReportsMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun +3 more
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Diphtheria toxin T-domain as a tool for inducing lipid vesicle fusion [PDF]
Communications ChemistryMembrane fusion is fundamental for many biological processes, including subcellular compartmentalization, cell growth, and exo- and endocytosis. It also plays a key role in the fabrication of artificial membrane structures, particularly vesicles. However,
Piotr Jasko +5 more
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Effects of a Pulse Electric Field on Electrofusion of Giant Unilamellar Vesicle (GUV)-Jurkat Cell
Journal of Thermal Science and Technology, 2012 Here we describe a new method to deliver membrane impermeable cryo-/lyo-protective agents (CPAs) into the cytosol of living cells via their electrofusion with Giant Unilamellar Vesicles (GUVs) containing large amounts of CPAs.
Ryo Shirakashi, Ulrich Zimmermann
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