Results 11 to 20 of about 3,495 (182)

Functional reconstitution of a voltage-gated potassium channel in giant unilamellar vesicles. [PDF]

open access: yesPLoS ONE, 2011
Voltage-gated ion channels are key players in cellular excitability. Recent studies suggest that their behavior can depend strongly on the membrane lipid composition and physical state. In vivo studies of membrane/channel and channel/channel interactions
Sophie Aimon   +5 more
doaj   +1 more source

Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos

open access: yesScientific Reports, 2022
Giant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes ...
Luana Teixeira Rodrigues Rossi   +7 more
doaj   +1 more source

Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.

open access: yesPLoS ONE, 2021
Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique ...
Malay Kumar Sarkar   +6 more
doaj   +1 more source

QS21-Initiated Fusion of Liposomal Small Unilamellar Vesicles to Form ALFQ Results in Concentration of Most of the Monophosphoryl Lipid A, QS21, and Cholesterol in Giant Unilamellar Vesicles

open access: yesPharmaceutics, 2023
Army Liposome Formulation with QS21 (ALFQ), a vaccine adjuvant preparation, comprises liposomes containing saturated phospholipids, with 55 mol% cholesterol relative to the phospholipids, and two adjuvants, monophosphoryl lipid A (MPLA) and QS21 saponin.
Erwin G. Abucayon   +3 more
doaj   +1 more source

Study on the Application of Electric Field to Giant Vesicles Comprised of 1,2-Dilauroyl-Sn-Glycero-3-Phosphatidylcholine Using Optical Fluorescence Microscopy [PDF]

open access: yesMaterials Research, 2017
The influence of alternating electric field (AC) in the structure and dynamics of giant unilamellar vesicles (GUVs) comprised of 1,2-dilauroyl-sn-glycero-3-phosphatidylcholine (DLPC) is reported.
Yasmine Miguel Serafini Micheletto   +7 more
doaj   +1 more source

Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening

open access: yesCHIMIA, 2022
Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic cells in their morphological characteristics because their architecture is precisely controllable.
Lukas Heuberger, Cornelia Palivan
doaj   +1 more source

Liposome-based liquid handling platform featuring addition, mixing, and aliquoting of femtoliter volumes. [PDF]

open access: yesPLoS ONE, 2014
This paper describes the utilization of giant unilamellar vesicles (GUVs) as a platform for handling chemical and biochemical reagents. GUVs with diameters of 5 to 10 µm and containing chemical/biochemical reagents together with inert polymers were fused
Hideaki Shiomi   +3 more
doaj   +1 more source

Effects of cholesterol on the size distribution and bending modulus of lipid vesicles.

open access: yesPLoS ONE, 2022
The influence of cholesterol fraction in the membranes of giant unilamellar vesicles (GUVs) on their size distributions and bending moduli has been investigated.
Mohammad Abu Sayem Karal   +6 more
doaj   +1 more source

GUV-Net for high fidelity shoeprint generation [PDF]

open access: yesComplex & Intelligent Systems, 2021
AbstractShoeprints contain valuable information for tracing evidence in forensic scenes, and they need to be generated into cleaned, sharp, and high-fidelity images. Most of the acquired shoeprints are found with low quality and/or in distorted forms. The high-fidelity shoeprint generation is of great significance in forensic science.
Muhammad Hassan   +6 more
openaire   +1 more source

A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles

open access: yesBio-Protocol, 2022
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen   +1 more
doaj   +1 more source

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