DNA Origami Assembly Inside Liposomes via Nanopore‐Mediated DNA Transportation
Assembly of DNA origami nanostructures inside giant unilamellar vesicles (GUVs) is demonstrated by supplying approximately 200 unique staple strands from the external environment.
Shoji Iwabuchi +5 more
doaj +2 more sources
Instant Membrane Stabilization by Damage-Triggered Actin Polymerization in Giant Unilamellar Vesicles. [PDF]
Damage‐triggered actin polymerization stabilizes membrane pores in giant unilamellar vesicles (GUVs). Membrane poration drives divalent cation influx, inducing rapid F‐actin assembly at the defect site. The resulting actin plug mechanically arrests pore expansion and preserves vesicle morphology, recapitulating the “plugging” phase of single‐cell ...
Nguyen HT, Lee SH, Kim CH, Shin K.
europepmc +2 more sources
Giant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes ...
Luana Teixeira Rodrigues Rossi +7 more
doaj +1 more source
Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid [PDF]
Caspase-8 is involved in death receptor-mediated apoptosis in type II cells, the proapoptotic programme of which is triggered by truncated Bid. Indeed, caspase-8 and Bid are the known intermediates of this signalling pathway.
AH Stegh +73 more
core +11 more sources
Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.
Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique ...
Malay Kumar Sarkar +6 more
doaj +1 more source
Army Liposome Formulation with QS21 (ALFQ), a vaccine adjuvant preparation, comprises liposomes containing saturated phospholipids, with 55 mol% cholesterol relative to the phospholipids, and two adjuvants, monophosphoryl lipid A (MPLA) and QS21 saponin.
Erwin G. Abucayon +3 more
doaj +1 more source
Study on the Application of Electric Field to Giant Vesicles Comprised of 1,2-Dilauroyl-Sn-Glycero-3-Phosphatidylcholine Using Optical Fluorescence Microscopy [PDF]
The influence of alternating electric field (AC) in the structure and dynamics of giant unilamellar vesicles (GUVs) comprised of 1,2-dilauroyl-sn-glycero-3-phosphatidylcholine (DLPC) is reported.
Yasmine Miguel Serafini Micheletto +7 more
doaj +1 more source
Liposome-based liquid handling platform featuring addition, mixing, and aliquoting of femtoliter volumes. [PDF]
This paper describes the utilization of giant unilamellar vesicles (GUVs) as a platform for handling chemical and biochemical reagents. GUVs with diameters of 5 to 10 µm and containing chemical/biochemical reagents together with inert polymers were fused
Hideaki Shiomi +3 more
doaj +1 more source
Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening
Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic cells in their morphological characteristics because their architecture is precisely controllable.
Lukas Heuberger, Cornelia Palivan
doaj +1 more source
Effects of cholesterol on the size distribution and bending modulus of lipid vesicles.
The influence of cholesterol fraction in the membranes of giant unilamellar vesicles (GUVs) on their size distributions and bending moduli has been investigated.
Mohammad Abu Sayem Karal +6 more
doaj +1 more source

