Results 31 to 40 of about 8,576 (213)
STAR Protocols, 2022 Summary: Here, we present a general protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles (GUVs). In this protocol, we use GUVs to mimic Ago1 protein’s phase separation behavior on the membrane through ...
Yuanxiang Zhu +3 more
doaj +1 more source
Influenza A matrix protein M1 induces lipid membrane deformation via protein multimerization [PDF]
, 2019 The matrix protein M1 of the Influenza A virus (IAV) is supposed to mediate viral assembly and budding at the plasma membrane (PM) of infected cells. In order for a new viral particle to form, the PM lipid bilayer has to bend into a vesicle toward the ...
Chiantia, Salvatore +2 more
core +1 more source
Angewandte Chemie, EarlyView.Current interest in artificial cell research underscores its potential to deepen our understanding of life's fundamental processes. This review highlights advances in bottom‐up coacervate‐based artificial cell engineering via combined integration of cellular hallmarks.
Arjan Hazegh Nikroo +3 more
wiley +2 more sources
, 2022 GUV preparation for membrane tube assay application
Liv Jensen, Chunmei Chang
openaire +1 more source
Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels
Bio-Protocol, 2020 Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris +2 more
doaj +1 more source
Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion
Membranes, 2023 Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special interest to researchers because of their similarity in size to eukaryotic cells. The most commonly used method for GUVs production is electroformation.
Zvonimir Boban +5 more
doaj +1 more source
On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis
, 2020 Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope.
Robinson, T. +2 more
core +1 more source
Membranes, 2021 Since its inception more than thirty years ago, electroformation has become the most commonly used method for growing giant unilamellar vesicles (GUVs). Although the method seems quite straightforward at first, researchers must consider the interplay of ...
Zvonimir Boban +3 more
doaj +1 more source
ESCRT-III mediated cell division in Sulfolobus acidocaldarius - a reconstitution perspective [PDF]
, 2014 In the framework of synthetic biology, it has become an intriguing question what would be the minimal representation of cell division machinery. Thus, it seems appropriate to compare how cell division is realized in different microorganisms. Inparticular,
Härtel, T., Schwille, P.
core +2 more sources
Microparticle assembly pathways on lipid membranes [PDF]
, 2017 Understanding interactions between microparticles and lipid membranes is of increasing importance, especially for unraveling the influence of microplastics on our health and environment.
Heinrich, Doris +2 more
core +3 more sources