Results 51 to 60 of about 3,495 (182)
DNA origami nanostructures functionalized with lipophilic anchors of distinct hydrophobicity reveal synergistic effects on membrane binding and phase‐specific localization. Weak anchors, although insufficient for binding alone, enhance membrane association and govern selective phase partitioning when combined with strong anchors, establishing multi ...
Subhasini Singh, Henri G. Franquelim
wiley +1 more source
Giant unilamellar vesicles (GUVs) are versatile cell models in biomedical and environmental research. Of the various GUV production methods, hydrogel-assisted GUV production is most easily implemented in a typical biological laboratory. To date, agarose,
Cherng-Wen Darren Tan +2 more
doaj +1 more source
Synthetic cells are positioned as enablers of green technology. But what is known about the environmental impact of synthetic cell production? In this review, we examine drivers of greenhouse gas emissions, toxicity, and other environmental impact categories of synthetic cell components.
Christina Hopf +2 more
wiley +1 more source
Repurposing a Lipid Droplet‐Associated Algal Protein as a Biosensor for Phosphatidic Acid
The DTH1 lipid binding domain (DTH1LBD) binds to lipid membranes containing phosphatidic acid (PA). Its N‐terminal amphipathic helices are sufficient for this association and, when labeled with solvatochromic fluorophores, can serve as in vitro PA biosensors.
Bilge Ercan +5 more
wiley +1 more source
Microfluidic Giant Polymer Vesicles Equipped with Biopores for High‐Throughput Screening of Bacteria
Understanding the mechanisms of antibiotic resistance is critical for the development of new therapeutics. Traditional methods for testing bacteria are often limited in their efficiency and reusability.
Lukas Heuberger +6 more
doaj +1 more source
Microfluidic characterization of biomimetic membrane mechanics with an on-chip micropipette
The mechanic properties of cell membranes control many biological processes. The complexity of natural membranes is often dealt with by building synthetic vesicles (Giant Unilamellar Vesicles, GUVs), which can be thought as micron-sized minimal cells ...
Marianne Elias +9 more
doaj +1 more source
Complex Phase Behavior of GUVs Containing Different Sphingomyelins [PDF]
Understanding the lateral organization of biological membranes plays a key role on the road to fully appreciate the physiological functions of this fundamental barrier between the inside and outside regions of a cell. Ternary lipid bilayers composed of a high and a low melting temperature lipid and cholesterol represent a model system that mimics some ...
Balleza D Mescola A MarínMedina N Ragazzini G Pieruccini M Facci P Alessandrini ABalleza D +6 more
openaire +3 more sources
Olikomycin A, a calcium‐dependent lipopeptide antibiotic, targets Gram‐positive bacteria by binding to phosphatidylglycerol and rapidly disrupting membrane integrity. Unlike daptomycin, it induces extensive membrane remodeling, vesicle deformation, and fragmentation.
Luisa Munz +4 more
wiley +1 more source
Electropermeabilization is a promising phenomenon that occurs when pulsed electric field with high frequency is applied to cells/vesicles. We quantify the required values of pulsed electric fields for the rupture of cell-sized giant unilamellar vesicles (
Md Kabir Ahamed +2 more
doaj +1 more source

