Results 71 to 80 of about 3,495 (182)

Electroformation of Giant Unilamellar Vesicles from Damp Films in Conditions Involving High Cholesterol Contents, Charged Lipids, and Saline Solutions

Membranes
Giant unilamellar vesicles (GUVs) are frequently used as membrane models in studies of membrane properties. They are most often produced using the electroformation method.
Ivan Mardešić, Zvonimir Boban, Marija Raguz   +2 more
doaj   +1 more source

Membrane Interaction of α-Synuclein in Different Aggregation States

Journal of Parkinson’s Disease, 2011
Aggregated α-synuclein in Lewy bodies is one of the hallmarks of Parkinson's disease (PD). Earlier observations of α-synuclein aggregates in neurons grafted into brains of PD patients suggested cell-to-cell transfer of α-synuclein and a prion-like ...
Marie Grey, Sara Linse, Hanna Nilsson, Patrik Brundin, Emma Sparr   +4 more
doaj   +1 more source

Dimensions, stability, and deformability of DOPC-cholesterol giant unilamellar vesicles formed by droplet transfer [version 2; peer review: 1 approved, 3 approved with reservations]

Open Research Europe
Background Understanding cell membrane-like lipid bilayers is crucial for studying fundamental biological mechanisms. Giant Unilamellar Vesicles (GUVs) are key tools for this investigation and have applications in both synthetic biology and, more ...
Stefano Palagi, Elisa Roberti, Elisa Linda Petrocelli, Dario Cecchi   +3 more
doaj   +1 more source

Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content

Membranes
Giant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić, Zvonimir Boban, Marija Raguz   +2 more
doaj   +1 more source

Lipid vesicle formation by encapsulation of SMALPs in surfactant-stabilised droplets

Heliyon
Understanding the intricate functions of membrane proteins is pivotal in cell biology and drug discovery. The composition of the cell membrane is highly complex, with different types of membrane proteins and lipid species.
Jorik Waeterschoot, Marta Barniol-Xicota, Steven Verhelst, Pieter Baatsen, Erin Koos, Jeroen Lammertyn, Xavier Casadevall i Solvas   +6 more
doaj   +1 more source

Large-Scale Fluorescence Microscopy Analysis of Lipid Membrane Conformational Changes Optimized and Enabled by an AI-Guided Image Detection Method

Journal of Experimental and Theoretical Analyses
Simplified and scalable models of physical systems are extremely valuable in a variety of different engineering fields to test and diagnose particular modes of failure and optimize build conditions.
Lillian Chang, Diya Devendiran, Julian Gard, Tiffany Gu, Annie Guan, Akira Yamamoto, Tapash Jay Sarkar, Edward Njoo, Joseph Pazzi   +8 more
doaj   +1 more source

Relationship between oligoarginine-induced membrane damage of single Escherichia coli cells and entry of the peptide into the cytoplasm

Biochemistry and Biophysics Reports
Cell-penetrating peptides (CPPs) can enter the cytosol of eukaryotic cells without killing them whereas some CPPs exhibit antimicrobial activity against bacterial cells.
Sabrina Sharmin, Md. Zahidul Islam, Masahito Yamazaki   +2 more
doaj   +1 more source

QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in Giant Unilamellar Vesicles. [PDF]

ACS Appl Mater Interfaces
Marshall Z, Bano R, Dylan P, Trifan L, Mckeaveney C, Gerber AP, Bae W.   +6 more
europepmc   +1 more source

Characterization of VPS13Cs binding to GUVs v1

VPS13C is a ER-lysosome contact site protein that transports lipids between the two organellar membranes and important for lysosomal membrane damage repair. We employed structure-function analysis of purified VPS13C and uncovered novel regulatory mechanisms.
Xinbo Wang, Dazhi Li, Pietro De Camilli, Karin Reinisch   +3 more
openaire   +1 more source

Protocol to study membrane-associated protein phase separation using protein-lipid blot overlay assays and giant unilamellar vesicles in vitro. [PDF]

STAR Protoc
Xie C, Wei W, You L, Cui X, Han ZG.
europepmc   +1 more source