Bulk Self-Assembly of Giant, Unilamellar Vesicles. [PDF]
ACS Nano, 2020 The desire to create cell-like models for fundamental science and applications has spurred extensive effort towards creating giant unilamellar vesicles (GUVs). However, a route to selectively self-assemble GUVs in bulk has remained elusive.
J. Kindt, J. Szostak, Anna Wang
semanticscholar +5 more sources
Scientific Reports, 2021 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate a rapid bi-directional movement of lipids without metabolic energy input. Here, we established a
Patricia P. M. Mathiassen +2 more
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Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]
Scientific ReportsMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun +3 more
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Diphtheria toxin T-domain as a tool for inducing lipid vesicle fusion [PDF]
Communications ChemistryMembrane fusion is fundamental for many biological processes, including subcellular compartmentalization, cell growth, and exo- and endocytosis. It also plays a key role in the fabrication of artificial membrane structures, particularly vesicles. However,
Piotr Jasko +5 more
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Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles
ACS Synthetic Biology, 2023 One of the main drivers within the field of bottom-up synthetic biology is to develop artificial chemical machines, perhaps even living systems, that have programmable functionality.
Pashiini Supramaniam +7 more
semanticscholar +5 more sources
Rapid Multi-Well Evaluation of Assorted Materials for Hydrogel-Assisted Giant Unilamellar Vesicle Production: Empowering Bottom-Up Synthetic Biology [PDF]
GelsGiant unilamellar vesicles (GUVs) are versatile cell models in biomedical and environmental research. Of the various GUV production methods, hydrogel-assisted GUV production is most easily implemented in a typical biological laboratory. To date, agarose,
Cherng-Wen Darren Tan +2 more
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Division and Regrowth of Phase‐Separated Giant Unilamellar Vesicles** [PDF]
Angewandte Chemie International Edition, 2020 Success in the bottom‐up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase‐separated giant unilamellar lipid vesicles (GUVs).
Yannik Dreher +4 more
semanticscholar +6 more sources
Towards Better Cell Membrane Mimics: Cholesterol-Containing Supported Lipid Bilayers on TiO2 [PDF]
Biophysical Journal, 2012 Podeu consultar la versió en castellà a: http://hdl.handle.net/11703/116989Podeu consultar la versió en francès a: http://hdl.handle.net/11703 ...
Zhu, Ling +2 more
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Effects of a Pulse Electric Field on Electrofusion of Giant Unilamellar Vesicle (GUV)-Jurkat Cell
Journal of Thermal Science and Technology, 2012 Here we describe a new method to deliver membrane impermeable cryo-/lyo-protective agents (CPAs) into the cytosol of living cells via their electrofusion with Giant Unilamellar Vesicles (GUVs) containing large amounts of CPAs.
Ryo SHIRAKASHI +4 more
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The impact of sphingomyelin and cholesterol on ordered lipid domain formation in the bovine milk fat globule membrane using artificial giant unilamellar vesicles as a model [PDF]
JDS CommunicationsGiant unilamellar vesicle (GUV) bilayers were constructed from polar lipids and cholesterol by electroformation as a model system to investigate the formation of ordered lipid domains (OLD) within the milk fat globule membrane (MFGM).
Haotian Zheng +2 more
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