Results 31 to 40 of about 19,800 (242)

Gel-Assisted Formation of Giant Unilamellar Vesicles [PDF]

Biophysical Journal, 2013
Giant unilamellar vesicles or GUVs are systems of choice as biomimetic models of cellular membranes. Although a variety of procedures exist for making single walled vesicles of tens of microns in size, the range of lipid compositions that can be used to grow GUVs by the conventional methods is quite limited, and many of the available methods involve ...
Weinberger, Andreas, Tsai, Feng-Ching, Koenderink, Gijsje H., Schmidt, Thais F., Itri, Rosângela, Meier, Wolfgang, Schmatko, Tatiana, Schröder, André, Marques, Carlos   +8 more
openaire   +3 more sources

Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network

BMC Bioinformatics, 2022
Background Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for analysis and calculating the desired information from the intensity traces.
Il-Hyung Lee, Sam Passaro, Selin Ozturk, Juan Ureña, Weitian Wang   +4 more
doaj   +1 more source

Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid [PDF]

, 2013
Caspase-8 is involved in death receptor-mediated apoptosis in type II cells, the proapoptotic programme of which is triggered by truncated Bid. Indeed, caspase-8 and Bid are the known intermediates of this signalling pathway.
AH Stegh, AH Stegh, AJ Garcia-Saez, AJ Garcia-Saez, AM Chinnaiyan, Ana-Jesus García-Sáez, B Apellaniz, B Leber, Beate Klösgen, Boris Zhivotovsky, C Scaffidi, D Ardail, D Ardail, D Chandra, D Marsh, D Marsh, D Needham, DW Chang, E Evans, E Krieghoff, E van den Brink-van der Laan, EE Varfolomeev, Eyal Gottlieb, F Gonzalvez, F Gonzalvez, F Gonzalvez, F Gonzalvez, FC Kischkel, François Gonzalvez, G Kroemer, GC Shearman, H Zhang, HR Stennicke, J Zha, Jesus Ayala-Sanmartin, JF Lovell, JH Li, JP Medema, K Milovic-Holm, KH Lee, KM Boatright, L Mathivet, Liberty François-Moutal, M Lamblet, M Lutter, M Lutter, M Muzio, M Muzio, MA Hughes, Mark Perry, MI Angelova, MO Hengartner, MP Boldin, N Keller, Olivier Jalmar, P Juo, P Ratanabanangkoon, Patrice X. Petit, Petra Schwille, PJ Jost, RF Epand, S Zhang, T Granjon, T Kuwana, T Parasassi, Thierry Granjon, VA Torres, VA Torres, W Helfrich, W Rawicz, WC Yeh, X Luo, Y Zaltsman, ZT Schug   +73 more
core   +9 more sources

A method for an efficient formation of giant vesicles of neutral phospholipids in microarrays using a microplotter equipment

Materials Research Express, 2021
A methodology which provides a high efficiency of giant vesicle formation was established using the gentle hydration method and a microplotter equipment.
Juan R Avendaño-Gomez, Obed A Solis-Gonzalez, Haydee González-Martínez, Miguel A Vega-Cuellar   +3 more
doaj   +1 more source

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles [PDF]

Journal of Visualized Experiments, 2017
The reshaping of the cell membrane is an integral part of many cellular phenomena, such as endocytosis, trafficking, the formation of filopodia, etc. Many different proteins associate with curved membranes because of their ability to sense or induce membrane curvature.
Prévost, Coline, Tsai, Feng-Ching, Bassereau, Patricia, Simunovic, Mijo   +3 more
openaire   +2 more sources

Thermodynamic relaxation drives expulsion in giant unilamellar vesicles [PDF]

Physical Biology, 2009
We investigated the thermodynamic relaxation of giant unilamellar vesicles (GUVs) which contained small vesicles within their interior. Quenching these vesicles from their fluid phase (T>Tm) through the phase transition in the gel state ...
Leirer, C. T., Wunderlich, B., Wixforth, Achim, Schneider, M. F.   +3 more
openaire   +4 more sources

Rheology of vesicle prototissues: A microfluidic approach

Frontiers in Physics, 2022
Synthetic biomimetic prototissues with reduced complexity can facilitate the understanding of intricate biological processes, by allowing the role of specific physical or chemical mechanisms to be isolated.
Majid Layachi, Laura Casas-Ferrer, Gladys Massiera, Laura Casanellas   +3 more
doaj   +1 more source

Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion

Membranes, 2023
Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special interest to researchers because of their similarity in size to eukaryotic cells. The most commonly used method for GUVs production is electroformation.
Zvonimir Boban, Ivan Mardešić, Sanja Perinović Jozić, Josipa Šumanovac, Witold Karol Subczynski, Marija Raguz   +5 more
doaj   +1 more source

Spontaneous Expulsion of Giant Lipid Vesicles Induced by Laser Tweezers [PDF]

, 1996
Irradiation of a giant unilamellar lipid bilayer vesicle with a focused laser spot leads to a tense pressurized state which persists indefinitely after laser shutoff.
Bar-Ziv, Roy, Moroz, J. David, Moses, Elisha, Nelson, Philip   +3 more
core   +3 more sources

Optimization of cryo-electron microscopy for quantitative analysis of lipid bilayers

Biophysical Reports, 2023
Cryogenic electron microscopy (cryo-EM) is among the most powerful tools available for interrogating nanoscale structure of biological materials.
Frederick A. Heberle, Doug Welsch, Haden L. Scott, M. Neal Waxham   +3 more
doaj   +1 more source