Communications Biology, 2021 By encapsulating proteins in giant unilamellar vesicles, Bashirzadeh et al find that actin crosslinkers, α-actinin and fascin, can self-assemble with actin into complex structures that depend on the degree of confinement.
Yashar Bashirzadeh +9 more
doaj +1 more source
Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid [PDF]
, 2013 Caspase-8 is involved in death receptor-mediated apoptosis in type II cells, the proapoptotic programme of which is triggered by truncated Bid. Indeed, caspase-8 and Bid are the known intermediates of this signalling pathway.
AH Stegh +73 more
core +4 more sources
Accurate reduced models for the pH oscillations in the urea-urease reaction confined to giant lipid vesicles [PDF]
J. Phys. Chem. B 127, 2955 (2023), 2022 This theoretical study concerns a pH oscillator based on the urea-urease reaction confined to giant lipid vesicles. Under suitable conditions, differential transport of urea and hydrogen ion across the unilamellar vesicle membrane periodically resets the pH clock that switches the system from acid to basic, resulting in self-sustained oscillations.
arxiv +1 more source
Construction and Design of Giant Unilamellar Vesicles [PDF]
, 2022 Giant Unilamellar Vesicles (GUV’s) consist of a phospholipid membrane bilayer and an aqueous space in between the membrane. Vesicles play a fundamental role in many fields of science; this is because you can incorporate different chemical compounds and ...
Frierson, Kory +2 more
core +1 more source
Mimicking Endocytosis Inside Giant Unilamellar Vesicles [PDF]
Biophysical Journal, 2012 Striving to further understanding of cellular processes and communication is one of the reasons for continuous progress in fields of synthetic biological systems, mimicking the functional components of cells. Through the controlled introduction of stimuli-responsive polymer into giant unilamellar vesicles (GUVs), we are able to represent macromolecular
Owe Orwar +3 more
openaire +2 more sources
Reconstitution of proteins on electroformed giant unilamellar vesicles [PDF]
, 2015 In vitro reconstitution of simplified biological systems from molecular parts has proven to be a powerful method for investigating the biochemical and biophysical principles underlying cellular processes. In recent years, there has been a growing interest in reconstitution of protein-membrane interactions to understand the critical role played by ...
Daniel A. Fletcher +2 more
openaire +2 more sources
Forming and loading giant unilamellar vesicles with acoustic jetting. [PDF]
Biomicrofluidics, 2020 Giant unilamellar vesicles (GUVs) are a useful platform for reconstituting and studying membrane-bound biological systems, offering reduced complexity compared to living cells. Several techniques exist to form GUVs and populate them with biomolecules of interest.
Armstrong M +3 more
europepmc +5 more sources
Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca2+ ions: A small-angle neutron scattering study [PDF]
, 2007 Dipalmitoylphosphatidylcholine (DPPC) was hydrated in 0.2-60 mM solution of CaCl 2 in heavy water and thoroughly homogenized by freezing-thawing process.
Almásy, László +3 more
core +1 more source
Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine. [PDF]
, 2015 Various protocell models have been constructed de novo with the bottom-up approach. Here we describe a silica-based protocell composed of a nanoporous amorphous silica core encapsulated within a lipid bilayer built by self-assembly that provides for ...
Brinker, C Jeffrey +3 more
core +5 more sources
On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis
, 2020 Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope.
Robinson, T. +2 more
core +1 more source