Results 71 to 80 of about 40,389 (237)

Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology [PDF]

, 2016
The quest to construct artificial cells from the bottom-up using simple building blocks has received much attention over recent decades and is one of the grand challenges in synthetic biology.
Elani, Y
core   +2 more sources

Microwave measurement of giant unilamellar vesicles in aqueous solution [PDF]

Scientific Reports, 2018
AbstractA microwave technique is demonstrated to measure floating giant unilamellar vesicle (GUV) membranes in a 25 μm wide and 18.8 μm high microfluidic channel. The measurement is conducted at 2.7 and 7.9 GHz, at which a split-ring resonator (SRR) operates at odd modes.
Yan Cui, William F. Delaney, Taghi Darroudi, Pingshan Wang   +3 more
openaire   +3 more sources

Active Membrane Fluctuations Studied by Micropipet Aspiration [PDF]

, 2001
We present a detailed analysis of the micropipet experiments recently reported in J-B. Manneville et al., Phys. Rev. Lett. 82, 4356--4359 (1999), including a derivation of the expected behaviour of the membrane tension as a function of the areal strain ...
A. N. Radionov, A. Royant, A.G. Petrov, D. Oesterhelt, E. Boroske, E. Evans, E. Evans, E. Pebay-Peyroula, F. Dumas, F. Faucon, F. Maingret, F. Maingret, G. Niggemann, H. J. Sass, H. Luecke, H. Luecke, J-B. Manneville, J. Heberle, J. Heberle, J. K. Lanyi, J. Prost, J. Prost, J. Prost, J.-B. Manneville, J.-L. Rigaud, M. Bloom, M. Bloom, M. I. Angelova, M. Jansen, M. Kummrow, M. M. Speretto, M. Seigneuret, M. Seigneuret, N. A. Dencher, O. G. Mouritsen, P. Bassereau, P. Méléard, Ph. Méléard, R. Henderson, R. Kwok, R. Lawaczeck, R. Lipowsky, S. Ramaswamy, S. Ramaswamy, S. Ramaswamy, S. Subramaniam, S. Subramaniam, T. Kouyama, U. Haupts, W. Helfrich, W. Kuhlbrandt, Y. Cao, Y. Kimura   +52 more
core   +2 more sources

Formation of Giant Unilamellar Vesicles Containing Active Proteins [PDF]

Biophysical Journal, 2014
Giant unilamellar vesicles (GUVs), composed of a phospholipid bilayer, are often used as a model for cell membranes. However the study of proteo-membrane interactions in this system is limited because the incorporation of integral and lipid-anchored proteins into the GUVs remains challenging.
Vladimir Adrien, Pincet Frederic, Andrea Gohlke, Isabelle Motta   +3 more
openaire   +2 more sources

Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking

Scientific Reports, 2018
Super-resolution imaging and single-particle tracking require cells to be immobile as any movement reduces the resolution of the measurements. Here, we present a method based on APTES-glutaraldehyde coating of glass surfaces to immobilize cells without ...
Łukasz Syga, Dian Spakman, Christiaan M. Punter, Bert Poolman   +3 more
doaj   +1 more source

Relaxation of a Simulated Lipid Bilayer Vesicle Compressed by an AFM [PDF]

, 2016
Using Coarse-Grained Molecular Dynamics simulations, we study the relaxation of bilayer vesicles, uniaxially compressed by an Atomic Force Microscope (AFM) cantilever. The relaxation time exhibits a strong force-dependence. Force-compression curves are very similar to recent experiments wherein giant unilamellar vesicles were compressed in a nearly ...
arxiv   +1 more source

Coupling between pore formation and phase separation in charged lipid membranes [PDF]

, 2015
We investigated the effect of charge on the membrane morphology of giant unilamellar vesicles (GUVs) composed of various mixtures containing charged lipids.
Hamada, Tsutomu, Higuchi, Yuji, Himeno, Hiroki, Ito, Hiroaki, Shimokawa, Naofumi, Takagi, Masahiro   +5 more
core   +2 more sources

Growth and shape transformations of giant phospholipid vesicles upon interaction with an aqueous oleic acid suspension [PDF]

Chem. Phys. Lipids 159 (2009) 67-76, 2009
The interaction of two types of vesicle systems was investigated: micrometer-sized, giant unilamellar vesicles (GUVs) formed from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and submicrometer-sized, large unilamellar vesicles (LUVs) formed from oleic acid and oleate, both in a buffered aqueous solution (pH=8.8).
arxiv   +1 more source

Transmembrane Activation of Catalysis and Protein Refolding in Synthetic Cells by Enzymes and Nanozymes

Advanced Functional Materials, EarlyView.
Synthetic cells are engineered herein to respond to an external chemical messenger by the activation of intracellular catalysis. The chemical messenger molecules are catalytically generated by an extracellular enzyme or a mineral surface, whereas the intracellular catalysis emerges via direct enzyme activation or via protein refolding.
Dante G. Andersen, Andreas B. Pedersen, Mireia Casanovas Montasell, Søren Moesgaard, Anja Bille Bohn, Alexander N. Zelikin   +5 more
wiley   +1 more source

Evaluating a Method for Producing Asymmetric Giant Unilamellar Vesicles [PDF]

Biophysical Journal, 2012
Giant unilamellar vesicles (GUVs) are important model systems for investigating physical properties of lipid bilayers. Recently, a method was developed called continuous droplet interface crossing encapsulation (cDICE)1, which creates GUVs by passing a stream of droplets through an oil water interface.
Gladys Massiera, Benjamin G. Horst, Sarah L. Keller, Manouk Abkarian, Matthew C. Blosser   +4 more
openaire   +2 more sources