Results 221 to 230 of about 51,146 (259)
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Polymer Encapsulation within Giant Lipid Vesicles
Langmuir, 2007We report encapsulation of polymers and small molecules within individual giant lipid vesicles (GVs; 3-80 microm), as determined by confocal fluorescence microscopy. Polymer-bound or free dyes were encapsulated within GVs by including these molecules in the aqueous solution during vesicle formation via gentle hydration.
Lisa M, Dominak, Christine D, Keating
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Membrane tensiometer for heavy giant vesicles
The European Physical Journal E, 2004One key parameter of giant-vesicles adhesion is their membrane tension, sigma. A theoretically simple but delicate way to impose (and measure) it is to use micropipette manipulation techniques. But usually, the vesicles are free and their tension is unknown, until an adhesion patch grows.
P-H, Puech, F, Brochard-Wyart
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Aqueous Phase Separation in Giant Vesicles
Journal of the American Chemical Society, 2002We report the synthesis and initial characterization of approximately 10 mum diameter lipid vesicles that contain two distinct aqueous phases. The aqueous two-phase system is a dextran/poly(ethylene glycol) solution that exhibits temperature-dependent phase behavior. Vesicles were prepared above the phase transition temperature of the polymer solution.
Helfrich, M. R. +4 more
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Microdomain evolution on giant unilamellar vesicles
Biomechanics and Modeling in Mechanobiology, 2012A chemo-mechanical model is used to capture the formation and evolution of microdomains on the deforming surface of giant unilamellar vesicles. The model is intended for the regime of vesicle dynamics characterized by a distinct difference in time scales between shape change and species transport.
Anand, Embar, John, Dolbow, Eliot, Fried
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Asymmetrical labeling of giant phospholipid vesicles
Pflügers Archiv - European Journal of Physiology, 2000The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent.
G, Gomiscek +4 more
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2019
This practical manual gives the reader a toolbox for starting work with giant vesicles, including expert's tips on proper preparation methods, measurement, and characterization methods. The contents build from a simple model to use of vesicles as advanced membrane and cell system models. It also includes fundamentals for understanding vesicle structure,
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This practical manual gives the reader a toolbox for starting work with giant vesicles, including expert's tips on proper preparation methods, measurement, and characterization methods. The contents build from a simple model to use of vesicles as advanced membrane and cell system models. It also includes fundamentals for understanding vesicle structure,
+6 more sources
Depth-Profiling with Giant Vesicle Membranes
Journal of the American Chemical Society, 2002A phospholipid fluorescent-labeled in one of three locations was paired with a phospholipid labeled with a quencher in one of three locations within a host phospholipid bilayer. The various combinations of quenching efficiencies allowed the location of the fluorescent labels to be determined.
Fredric M, Menger +2 more
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Formation of tubules and giant vesicles from large multilamellar vesicles
Journal of Colloid and Interface Science, 2003This study reports an observation of submicrometer multilamellar vesicles (MLVs) prepared by simply freeze-thawing a phospholipid dispersion at full hydration that transformed into giant vesicles (GVs) and tubules (TUs) when confined between microscope glass slides.
Douliez, Jean-Paul +2 more
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Collapse of giant phosphatidylcholine vesicles
Chemistry and Physics of Lipids, 1996Abstract The collapse of giant phosphatidylcholine vesicles was recorded by video microscopy. The vesicle membranes transform into small particles which seem to represent a multiply self-connected bilayer, possibly a cubic phase. We estimate the lipid density of the black (or bright) particles and the lateral tension rupturing the vesicle bilayers.
M. Kummrow, W. Helfrich
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Rapid Electroformation of Giant Vesicles
Chemistry Letters, 2011Abstract With a small amount of an electrolyte, electroformation of giant vesicles (GVs) proceeded faster than in pure water. In 50 µM neutral phosphate buffer, a GV larger than 50 µm formed in 100 s. The time required for the completion was 5–10 min while electroformation in pure water usually takes 90–120 min.
Yukihisa Okumura, Koji Urita
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