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Giant vesicles as cell models

Integrative Biology, 2012
Tremendous progress has been made in recent years in understanding the working of the living cell, including its micro-anatomy, signalling networks, and regulation of genes. However, an understanding of cellular phenomena using fundamental laws starting from first principles is still very far away.
Fenz, S.F., Sengupta, K.
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Protein Reconstitution Inside Giant Unilamellar Vesicles

Annual Review of Biophysics, 2021
Giant unilamellar vesicles (GUVs) have gained great popularity as mimicries for cellular membranes. As their sizes are comfortably above the optical resolution limit, and their lipid composition is easily controlled, they are ideal for quantitative light microscopic investigation of dynamic processes in and on membranes.
Litschel, T., Schwille, P.
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Giant Vesicles: Preparations and Applications

ChemBioChem, 2010
AbstractThere is considerable interest in preparing cell‐sized giant unilamellar vesicles from natural or nonnatural amphiphiles because a giant vesicle membrane resembles the self‐closed lipid matrix of the plasma membrane of all biological cells. Currently, giant vesicles are applied to investigate certain aspects of biomembranes.
Walde P.   +3 more
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Giant Vesicles with Membranous Microcompartments

Langmuir, 2011
Incubation of a cell-sized lipid membrane vesicle (giant vesicle, GV) in a diluted aqueous solution of neutral phosphate buffer salts or glucose transformed the GV to an oligovesicular vesicle (OVV) that encapsulates one or more smaller GVs. During the incubation, the membrane of flaccid vesicle invaginated and closed to form the inner vesicle of an ...
Yukihisa, Okumura   +3 more
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Asymmetric Giant Lipid Vesicle Fabrication

2014
Synthetic lipid bilayers have long been used as models of cell membranes. The compositional asymmetry in the eukaryotic plasma membrane is a key chemical characteristic of this membrane that has traditionally been difficult to reproduce in synthetic systems.
Peichi C, Hu, Noah, Malmstadt
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Dynamics of Giant Vesicles

Molecular Crystals and Liquid Crystals Science and Technology. Section A. Molecular Crystals and Liquid Crystals, 1997
Abstract The dynamics of vesicle shape transformations are governed by the competition between curvature energy, geometrical constraints and viscous dissipation in the surrounding liquid. After a discussion of the general principles, three examples illustrate these concepts, (i) Slow conformal diffusion of vesicles of higher genus arises from the ...
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Giant Seminal Vesicle Stones

Urologia Internationalis, 2002
Seminal vesicle stones are rare conditions and only a few cases have been reported in the literature. Here we present 2 cases with single and multiple seminal vesicle calculi.
Mete Kilciler   +5 more
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Properties of giant vesicles

Current Opinion in Colloid & Interface Science, 2000
We have discussed the specific properties of giant vesicles and their use as model systems for fluid interfaces and biomembranes. Recent advances in giant vesicle research include systematic measurements of visco-elastic parameters as a function of membrane composition, experiments with water-soluble amphiphiles and active membranes, as well as the ...
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Polymer Encapsulation within Giant Lipid Vesicles

Langmuir, 2007
We report encapsulation of polymers and small molecules within individual giant lipid vesicles (GVs; 3-80 microm), as determined by confocal fluorescence microscopy. Polymer-bound or free dyes were encapsulated within GVs by including these molecules in the aqueous solution during vesicle formation via gentle hydration.
Lisa M, Dominak, Christine D, Keating
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Membrane tensiometer for heavy giant vesicles

The European Physical Journal E, 2004
One key parameter of giant-vesicles adhesion is their membrane tension, sigma. A theoretically simple but delicate way to impose (and measure) it is to use micropipette manipulation techniques. But usually, the vesicles are free and their tension is unknown, until an adhesion patch grows.
P-H, Puech, F, Brochard-Wyart
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