Results 41 to 50 of about 268,225 (293)

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Generation and characterization of three isogenic induced pluripotent stem cell lines from a patient with Bardet-Biedl syndrome and homozygous for the BBS5 variant

Stem Cell Research, 2019
Bardet-Biedl syndrome (BBS), an autosomal recessive disease, is associated with non-functional primary cilia. BBS5 is part of the protein complex termed the BBSome.
Caroline Amalie Brunbjerg Hey, Lasse Jonsgaard Larsen, Zeynep Tümer, Karen Brøndum-Nielsen, Karen Grønskov, Tina Duelund Hjortshøj, Lisbeth Birk Møller   +6 more
doaj   +1 more source

The Utilization of Linear Polylysine Coupled with Mechanic Forces to Extract Microbial DNA from Different Matrices

Microorganisms, 2020
Molecular approaches are powerful tools that are used for medical or environmental diagnoses. However, the main limitations of such a tools are that they extract low levels of DNA and they do not remove the inhibitors of polymerase chain reaction (PCR ...
Celia François, Celia Martinez, Clement Faye, Nathalie Pansu, Catherine Dunyach-Remy, Laurent Garrelly, Benoit Roig, Axelle Cadiere   +7 more
doaj   +1 more source

Geomorphological development of aquatic mesohabitats in shore channels along longitudinal training dams

Remote Sensing in Ecology and Conservation, 2022
Longitudinal training dams (LTDs) are novel hydraulic engineering structures in the river Waal intended to facilitate intensive navigation and safe discharges in the main channel while providing sheltered habitats for aquatic biota in shore channels ...
Natasha Y. Flores, Frank P. L. Collas, Rob S. E. W. Leuven   +2 more
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

On the relationship between climate sensitivity and modelling uncertainty

Tellus: Series A, Dynamic Meteorology and Oceanography, 2017
Climate model projections are used to investigate the potential impacts of climate change on future weather, agriculture, water resources, human health, the global economy, etc. However, climate projections have a broad range of associated uncertainties,
Cecilie Mauritzen, Tatjana Zivkovic, Vidyunmala Veldore   +2 more
doaj   +1 more source

Characterization of Lignins from Sugarcane Bagasse Pretreated with Green Liquor Combined with Ethanol and Hydrogen Peroxide

BioResources, 2016
Sugarcane bagasse was pretreated by green liquor combined with ethanol (GL-Ethanol) and green liquor combined with H2O2 (GL-H2O2). After 72 h of enzymatic hydrolysis, the glucose yields of sugarcane bagasse pretreated with GL-Ethanol and GL-H2O2 were 97 ...
Ziyuan Zhou, Yi Cheng, Weiming Zhang, Jianxin Jiang, Fuhou Lei   +4 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source