Results 71 to 80 of about 1,513,313 (316)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Superanalogs of the Calogero operators and Jack polynomials

, 2000
A depending on a complex parameter $k$ superanalog ${\mathcal S}{\mathcal L}$ of Calogero operator is constructed; it is related with the root system of the Lie superalgebra ${\mathfrak{gl}}(n|m)$.
Chalykh O, Dixmier J, Kerov S, Lapoint L, Macdonald I, Olshanetsky M, Sergeev A N, Stanley R, Veselov A   +8 more
core   +2 more sources

EFFICIENCY OF FUNGICIDES FOR OIDIUM CONTROL IN SOYBEAN CROP

Ciência Agrícola, 2019
One of the most relevant factors in soybean productivity is fungal diseases such as oidium (Microsphaera diffusa), which can be controlled through resistant cultivars or preventive control through the application of fungicides.
Matheus da Silva Maciel, Gabriel Fabiani, Jesiomar Delazeri, Douglas Mello Praetorius, Valter Pivotto Bussolotto, Rafael Goulart Machado, José de Alencar Lemos Vieira Junior, Leonita Beatriz Girardi, Katia Trevizan, Ronaldo Elias, Volmir Atilio Farina   +10 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Partial synchronization of the colonial diatom Bacillaria "paradoxa" [PDF]

Research Ideas and Outcomes, 2016
Richard Gordon
doaj   +2 more sources

Identification of the Uncertainties for the Calibration of the Partial Safety Factors for Load in Tidal Turbines

Journal of Marine Science and Engineering, 2016
Tidal energy is nowadays one of the fastest growing types of marine renewable energy. In particular, Horizontal Axis Tidal Turbines (HATTs) are the most advanced designs and the most appropriate for standardization. This paper presents a review of actual
Gaizka Zarraonandia Simeón, Claudio Bittencourt Ferreira   +1 more
doaj   +1 more source

Remote Monitoring in Myasthenia Gravis: Exploring Symptom Variability

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Myasthenia gravis (MG) is a rare, autoimmune disorder characterized by fluctuating muscle weakness and potential life‐threatening crises. While continuous specialized care is essential, access barriers often delay timely interventions. To address this, we developed MyaLink, a telemedical platform for MG patients.
Maike Stein, Maximilian Mönch, Meret Herdick, Frauke Stascheit, Sarah Hoffmann, Hannah Preßler, Philipp Mergenthaler, Carla Dusemund, Paolo Doksani, Haoqi Sun, Pushpa Narayanaswami, Andreas Meisel, Lea Gerischer, Sophie Lehnerer   +13 more
wiley   +1 more source

To b(gl) or not to b(gl): how cryptic are “cryptic” genes? [PDF]

Journal of Biosciences, 1998
A striking feature that has emerged from the genome sequencing projects that have been completed so far is the extremely high coding percentage seen in all bacterial genomes. In the case of Escherichia coli, it is higher than 90% (in higher eukaryotes including humans, it can be as low as 2%). Despite running such a tight ship, many prokaryotic genomes
openaire   +2 more sources

AI-driven sustainability marketing transforming consumers’ perception toward eco-friendly brands

Discover Sustainability
Artificial intelligence [AI] is transforming sustainability marketing by influencing consumer behaviour. This is because of the in terms of engagement with environmentally friendly brands due to increasing raising environmental consciousness and ...
Vikas Garg, Sailaja Bohara, Arpita Srivastav   +2 more
doaj   +1 more source