Results 41 to 50 of about 151,779 (318)

PCA‐Based Database Mining Enables the Discovery of Bacterial Carbene Transferases for Stereodivergent Cyclopropanation

Angewandte Chemie, EarlyView.
Database mining using principal component analysis (PCA)‐based clustering enabled discovery of bacterial enzymes capable of catalyzing stereodivergent cyclopropanation of styrene. Statistical analyses of sequence data further revealed characteristic structural properties of these enzymes, highlighting the potential of the bioinformatics tools for ...
Shunsuke Kato, Koki Takeuchi, Kohei Umeda, Hisashi Kudo, Tomohisa Hasunuma, Takashi Hayashi   +5 more
wiley   +2 more sources

Imbalanced globin chain synthesis determines erythroid cell pathology in thalassemic mice

Haematologica, 2009
Background β-thalassemia occurs from the imbalanced globin chain synthesis due to the absence or inadequate β-globin chain production. The excessive unbound α-globin chains precipitate in erythroid precursors and mature red blood cells leading to ...
Kanitta Srinoun, Saovaros Svasti, Worrakavee Chumworathayee, Jim Vadolas, Phantip Vattanaviboon, Suthat Fucharoen, Pranee Winichagoon   +6 more
doaj   +1 more source

βT87Q-Globin Gene Therapy Reduces Sickle Hemoglobin Production, Allowing for Ex Vivo Anti-sickling Activity in Human Erythroid Cells

Molecular Therapy: Methods & Clinical Development, 2020
Lentiviral addition of βT87Q-globin, a modified β-globin with an anti-sickling mutation, is currently being used in gene therapy trials for sickle cell disease (SCD) and β-thalassemia patients.
Selami Demirci, Bjorg Gudmundsdottir, Quan Li, Juan J. Haro-Mora, Tina Nassehi, Claire Drysdale, Morgan Yapundich, Jackson Gamer, Fayaz Seifuddin, John F. Tisdale, Naoya Uchida   +10 more
doaj   +1 more source

Introduction and Expression of a Rabbit β-globin Gene in Mouse Fibroblasts [PDF]

, 1979
The cloned chromosomal rabbit ß-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20
Axel, R., Lacy, E., Maniatis, T., Silverstein, S., Wigler, M., Wold, B.   +5 more
core  

Regulated expression of human A γ-, β-, and hybrid γ β-globin genes in transgenic mice: manipulation of the developmental expression patterns. [PDF]

, 1986
We have introduced the human fetal gamma- and adult beta-globin genes into the germ line of mice. Analysis of the resulting transgenic mice shows that the human gamma-globin gene is expressed like an embryonic mouse globin gene; the human beta-globin ...
Grosveld, F.G. (Frank), Hurst, J., Kollias, G. (George), Wrighton, N.   +3 more
core   +1 more source

TMC4 localizes to multiple taste cell types in the mouse taste papillae

FEBS Open Bio, EarlyView.
Transmembrane channel‐like 4 (TMC4), a voltage‐dependent chloride channel, plays a critical role in amiloride‐insensitive salty taste transduction. TMC4 is broadly expressed in all mature taste cell types, suggesting a possible involvement of multiple cell types in this pathway.
Momo Murata, Yoshikazu Saito, Yoichi Kasahara, Wataru Fujii, Takumi Misaka, Tomiko Asakura, Masataka Narukawa   +6 more
wiley   +1 more source

Globin-based redox signaling [PDF]

Worm, 2016
In recent years, moderate levels of reactive oxygen species (ROS) have become recognized as signaling cues that participate at all levels of cellular organization. Globins, with their redox-active heme iron and ubiquitous presence, seem ideally suited to participate in ROS metabolism.
De Henau, Sasha, Braeckman, Bart
openaire   +3 more sources

Structural interpretation of the amino acid sequence of a second domain from the Artemia covalent polymer globin [PDF]

, 1990
Artemia has a complex extracellular hemoglobin of Mr 260,000 comprising two globin chains (Mr 130,000) each of which is a polymer of eight covalently linked domains of Mr 16,000.
Alard, Philippe, De Smet, Koen, Moens, Luc, Trotman, Clive, Van Beeumen, Jozef, Van de Peer, Yves, Van Hauwaert, Marie-Louise, Ver Donck, Kris, Wodak, Shoshana   +8 more
core   +1 more source

Metallofullerenol Sc3N@C80(OH)18: A New Generation Radioprotector Protecting Human Erythrocytes Against Multiple Biochemical Damage Modes Upon Gamma Irradiation, Identifying It as a Scavenger of Short‐ and Long‐Lived Radicals

Advanced Healthcare Materials, EarlyView.
Metallofullerenol Sc3N@C80(OH)18 demonstrates strong radioprotective properties as a scavenger of both short‐ and long‐lived radicals. The study reveals protection of human erythrocytes from γ‐radiation–induced biochemical damage via post‐irradiation removal of primary and secondary reactive oxidants, supported by pulse radiolysis kinetics.
Jacek Grebowski, Maciej Studzian, Szymon Lekki‐Porebski, Anna Konarska, Marian Wolszczak, Grzegorz Litwinienko, Lukasz Pulaski   +6 more
wiley   +1 more source

Formation of the Native Topology of a Protein is due to the “Conserved but Non-Functional” Residues: A Case of Apomyoglobin Folding

Frontiers in Bioscience-Landmark
This paper is dedicated to the memory of Oleg B. Ptitsyn (1929-1999) and presents an answer to his question: “What is the role of conserved non-functional residues in protein folding?”. This answer follows from the experimental works of three labs.
Valentina E. Bychkova, Dmitry A. Dolgikh, Vitalii A. Balobanov, Alexei V. Finkelstein   +3 more
doaj   +1 more source