Results 61 to 70 of about 74,517 (259)

Enhancer‐dependent gene regulation in space, time, and malignancies

International Journal of Cancer, EarlyView.
Abstract Control of cell‐type‐specific gene activation requires the coordinated activity of distal regulatory elements, including enhancers, whose inputs must be temporally integrated. Dysregulation of this regulatory capacity, such as aberrant usage of enhancers, can result in malignant transformation of cells.
Belinda Blum, Victoria Dachtler, Angelika Feldmann   +2 more
wiley   +1 more source

Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells. [PDF]

PLoS ONE, 2011
Gene therapy for sickle cell disease will require efficient delivery of a tightly regulated and stably expressed gene product to provide an effective therapy. In this study we utilized the non-viral Sleeping Beauty (SB) transposon system using the SB100X
Lucas M Sjeklocha, Chang-Won Park, Phillip Y-P Wong, Mark J Roney, John D Belcher, Dan S Kaufman, Gregory M Vercellotti, Robert P Hebbel, Clifford J Steer   +8 more
doaj   +1 more source

CTCF-mediated transcriptional regulation through cell type-specific chromosome organization in the β-globin locus [PDF]

, 2012
The principles underlying the architectural landscape of chromatin beyond the nucleosome level in living cells remains largely unknown despite its potential to play a role in mammalian gene regulation. We investigated the 3-dimensional folding of a 1 Mbp region of human chromosome 11 containing the {\beta}-globin genes by integrating looping ...
arxiv   +1 more source

Quantitation of human gamma globin genes and gamma globin mRNA with purified gamma globin complementary DNA. [PDF]

Journal of Clinical Investigation, 1976
Complementary DNA (cDNA) specific for gamma-globin nucleotide sequences has been prepared by hybridizing total cDNA made from cord blood messenger RNA (mRNA) as template to an excess of normal adult human globin mRNA and recovering the single-stranded cDNA from hydroxylapatite.
Clayton Natta, Arthur Bank, Francesco Ramirez, Joyce V. O'Donnell   +3 more
openaire   +3 more sources

Improved cervical screening using HPV type restriction and cycle threshold limit setting with the AmpFire assay: A prospective screening cohort of women with and without HIV in Botswana

International Journal of Gynecology &Obstetrics, EarlyView.
Abstract Objective The aim of this study was to evaluate the performance of HPV type restriction and cycle threshold (Ct)‐limit setting to optimize detection of cervical intraepithelial neoplasia (CIN) with primary HPV testing. Methods Baseline cervical screening at time of entry into a prospective longitudinal cohort of women with and without HIV was ...
Rebecca Luckett, Doreen Ramogola‐Masire, Sikhulile Moyo, Annika Gompers, Anna Modest, Natasha Moraka, Thanolo Kashamba, Leabaneng Tawe, Farzad Noubary, Louise Kuhn, Surbhi Grover, Scott Dryden‐Peterson, Greta Dreyer, Joseph Makhema, Matthys H. Botha, Michele R. Hacker, Roger Shapiro   +16 more
wiley   +1 more source

Ectopic MYBL2-Mediated Regulation of Androglobin Gene Expression

Cells
Androglobin (ADGB) is a highly conserved and recently identified member of the globin superfamily. Although previous studies revealed a link to ciliogenesis and an involvement in murine spermatogenesis, its physiological function remains mostly unknown ...
Antonia Herwig, Carina Osterhof, Anna Keppner, Darko Maric, Teng Wei Koay, Ambre Mbemba-Nsungi, David Hoogewijs   +6 more
doaj   +1 more source

The diversity of globin‐coupled sensors [PDF]

FEBS Letters, 2003
The recently discovered globin‐coupled sensors (GCSs) are heme‐containing two‐domain transducers distinct from the PAS domain superfamily. We have identified an additional 22 GCSs with varying multi‐domain C‐terminal transmitters through a search of the complete and incomplete microbial genome datasets.
Maqsudul Alam, Shaobin Hou, Tracey Allen K. Freitas   +2 more
openaire   +3 more sources

Mitochondrial tRNAGlu 14687A>G May Be A Novel Mutation for Type 2 Diabetes Mellitus

Journal of Clinical Laboratory Analysis, EarlyView.
We identified a novel mitochondrial tRNAGlu 14687A>G mutation in a Han Chinese pedigree with diabetes; the m.14687A>G mutation occurred at a conserved nucleotide of tRNAGlu, which was important for tRNA structure and function. Using a cybrid cell model, we found that mutant cells exhibited much more severe mitochondrial dysfunctions than control cells,
Xiaojuan Rao, Liran Xie, Shuangwei Shi, Yifan Fang   +3 more
wiley   +1 more source

CRISPR-Cas9 interrogation of a putative fetal globin repressor in human erythroid cells.

PLoS ONE, 2019
Sickle Cell Disease and ß-thalassemia, which are caused by defective or deficient adult ß-globin (HBB) respectively, are the most common serious genetic blood diseases in the world. Persistent expression of the fetal ß-like globin, also known as 𝛾-globin,
Jennifer E Chung, Wendy Magis, Jonathan Vu, Seok-Jin Heo, Kirmo Wartiovaara, Mark C Walters, Ryo Kurita, Yukio Nakamura, Dario Boffelli, David I K Martin, Jacob E Corn, Mark A DeWitt   +11 more
doaj   +1 more source

Decrease of alpha-chains in beta-thalassemia

Thalassemia Reports, 2013
In the pathophysiology of beta-thalassemia, globin chain imbalance plays a central role in predicting red blood cell (RBC) life span and disease severity. Strategies to improve globin chain imbalance are therefore a legitimate target in the management of
M. Papadaki, George Vassilopoulos
doaj   +1 more source