Construction of an amylolytic Saccharomyces cerevisiae strain with high copies of α-amylase and glucoamylase genes integration for bioethanol production from sweet potato residue [PDF]
Frontiers in MicrobiologySweet potato residue (SPR) is the by-product of starch extraction from fresh sweet potatoes and is rich in carbohydrates, making it a suitable substrate for bioethanol production.
Xin Wang +18 more
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Safety evaluation of the food enzyme glucan 1,4‐α‐glucosidase from the genetically modified Trichoderma reesei strain DP‐Nzh109 [PDF]
EFSA JournalThe food enzyme glucan 1,4‐α‐glucosidase (4‐α‐d‐glucan glucohydrolase; EC 3.2.1.3) is produced with the genetically modified Trichoderma reesei strain DP‐Nzh109 by Genencor International B.V. The genetic modifications do not give rise to safety concerns.
EFSA Panel on Food Enzymes (FEZ) +16 more
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The effect of polyhydroxylated alkaloids on maltase-glucoamylase. [PDF]
PLoS ONE, 2013 One of the most important carbohydrate-splitting enzymes is themaltase-glucoamylase which catalyzes the hydrolysis of alpha-glucosidic linkages. Maltase-glucoamylase inhibitors during the last few years have aroused medical interests in the treatment of ...
Qian Shang +4 more
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Temperature-triggered enzyme immobilization and release based on cross-linked gelatin nanoparticles. [PDF]
PLoS ONE, 2012 A glucoamylase-immobilized system based on cross-linked gelatin nanoparticles (CLGNs) was prepared by coacervation method. This system exhibited characteristics of temperature-triggered phase transition, which could be used for enzyme immobilization and ...
Zhenhai Gan +3 more
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Studies on mammalian glucoamylases with special reference to monkey intestinal glucoamylase [PDF]
Biochemical Journal, 1970 1. Highly purified preparations of glucoamylase were obtained from liver, spleen and intestine of the monkey. The enrichment factor was lower for intestine (60-fold) compared with that of liver (1200-fold) and of spleen (2000-fold) but the final specific activities were of a similar magnitude. 2. The liver and spleen enzymes had maximum activity at pH4.
B, Seetharam +2 more
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Optimization and application of Miqu prepared by Rhizopus oryzae from medium-high temperature Daqu
Shipin yu jixie, 2022 Objective: A microbial enhancer with high-yield glucoamylase was developed from medium-high temperature Daqu and applied to the brewing of Daqu Baijiu for the yield increasing.
HUANG Ke-yi +5 more
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Immobilization of glucoamylase on macroporous spheres [PDF]
Acta Periodica Technologica, 2004 Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent.
Milosavić Nenad B. +3 more
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Biotechnology for Biofuels, 2021 Background Glucoamylase is an important industrial enzyme in the saccharification of starch into glucose. However, its poor thermostability and low catalytic efficiency limit its industrial saccharification applications.
Lige Tong +11 more
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Subsite structure of Chalara paradoxa glucoamylase and interaction of the glucoamylase with cyclodextrins. [PDF]
Agricultural and Biological Chemistry, 1989 The action of Chalara paradoxa glucoamylase (raw-starch-digesting enzyme) was studied with linear and cyclic maltodextrins. Subsite affinities (Ai) of the amylase were evaluated by the subsite theory. The active site was considered to be made up of seven subsites: = 0.05 kcal/mol,,42= 4.99 kcal/mol, A3 = 1.30 kcal/mol, A4 = 0.77 kcal/mol, A5 =0.33 kcal/
MONMA, Mitsuru +2 more
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Biotechnology for Biofuels, 2021 Background Starch is one of the most important renewable polysaccharides in nature for production of bio-ethanol. The starch saccharification step facilitates the depolymerization of starch to yield glucose for biofuels production. The filamentous fungus
Wenzhu Guo +9 more
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